Recombinant Full Length Human OPLAH Protein, C-Flag-tagged

• Cat.No.: OPLAH-1898HFL
• Product Overview: Recombinant Full Length Human OPLAH Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD).
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 137.3 kDa
• AA Sequence: MGSPEGRFHFAIDRGGTFTDVFAQC PGGHVRVLKLLSEDPANYADAPTEG IRRILEQEAGMLLPRDQPLD SSHIASIRMGTTVATNALLERKGER VALLVTRGFRDLLHIGTQARGDLFD LAVPMPEVLYEEVLEVDERV VLHRGEAGTGTPVKGRTGDLLEVQQ PVDLGALRGKLEGLLSRGIRSLAVV LMHSYTWAQHEQQVGVLARE LGFTHVSLSSEAMPMVRIVPRGHTA CADAYLTPAIQRYVQGFCRGFQGQL KDVQVLFMRSDGGLAPMDTF SGSSAVLSGPAGGVVGYSATTYQQE GGQPVIGFDMGGTSTDVSRYAGEFE HVFEASTAGVTLQAPQLDIN TVAAGGGSRLFFRSGLFVVGPESAG AHPGPACYRKGGPVTVTDANLVLGR LLPASFPCIFGPGENQPLSP EASRKALEAVATEVNSFLTNGPCPA SPLSLEEVAMGFVRVANEAMCRPIR ALTQARGHDPSAHVLACFGG AGGQHACAIARALGMDTVHIHRHSG LLSALGLALADVVHEAQEPCSLLYA PETFVQLDQRLSRLEEQCVD ALQAQGFPRSQISTESFLHLRYQGT DCALMVSAHQHPATARSPRAGDFGA AFVERYMREFGFVIPERPVV VDDVRVRGTGRSGLRLEDAPKAQTG PPRVDKMTQCYFEGGYQETPVYLLA ELGYGHKLHGPCLIIDSNST ILVEPGCQAEVTKTGDICISVGAEV PGTVGPQLDPIQLSIFSHRFMSIAE QMGRILQRTAISTNIKERLD FSCALFGPDGGLVSNAPHIPVHLGA MQETVQFQIQHLGADLHPGDVLLSN HPSAGGSHLPDLTVITPVFW PGQTRPVFYVASRGHHADIGGITPG SMPPHSTMLQQEGAVFLSFKLVQGG VFQEEAVTEALRAPGKVPNC SGTRNLHDNLSDLRAQVAANQKGIQ LVGELIGQYGLDVVQAYMGHIQANA ELAVRDMLRAFGTSRQARGL PLEVSSEDHMDDGSPIRLRVQISLS QGSAVFDFSGTGPEVFGNLNAPRAV TLSALIYCLRCLVGRDIPLN QGCLAPVRVVIPRGSILDPSPEAAV VGGNVLTSQRVVDVILGAFGACAAS QGCMNNVTLGNAHMGYYETV AGGAGAGPSWHGRSGVHSHMTNTRI TDPEILESRYPVILRRFELRRGSGG RGRFRGGDGVTRELLFREEA LLSVLTERRAFRPYGLHGGEPGARG LNLLIRKNGRTVNLGGKTSVTVYPG DVFCLHTPGGGGYGDPEDPA PPPGSPPQALAFPEHGSVYEYRRAQ EAV SGPTRTRRLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Glutathione metabolism

Gene Information

• Gene Name: OPLAH 5-oxoprolinase, ATP-hydrolysing [ Homo sapiens (human) ]
• Official Symbol: OPLAH
• Synonyms: OPLA; OPLAHD; 5-Opase
• Gene ID: 26873
• mRNA Refseq: NM_017570.5
• Protein Refseq: NP_060040.1
• MIM: 614243
• UniProt ID: O14841