Recombinant Full Length Human PAPPA Protein, C-Flag-tagged

• Cat.No.: PAPPA-1798HFL
• Product Overview: Recombinant Full Length Human PAPPA Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 178.6 kDa
• AA Sequence: MRLWSWVLHLGLLSAALGCGLAERP RRARRDPRAGRPPRPAAGPATCATR AARGRRASPPPPPPPGGAWE AVRVPRRRQQREARGATEEPSPPSR ALYFSGRGEQLRLRADLELPRDAFT LQVWLRAEGGQRSPAVITGL YDKCSYISRDRGWVVGIHTISDQDN KDPRYFFSLKTDRARQVTTINAHRS YLPGQWVYLAATYDGQFMKL YVNGAQVATSGEQVGGIFSPLTQKC KVLMLGGSALNHNYRGYIEHFSLWK VARTQREILSDMETHGAHTA LPQLLLQENWDNVKHAWSPMKDGSS PKVEFSNAHGFLLDTSLEPPLCGQT LCDNTEVIASYNQLSSFRQP KVVRYRVVNLYEDDHKNPTVTREQV DFQHHQLAEAFKQYNISWELDVLEV SNSSLRRRLILANCDISKIG DENCDPECNHTLTGHDGGDCRHLRH PAFVKKQHNGVCDMDCNYERFNFDG GECCDPEITNVTQTCFDPDS PHRAYLDVNELKNILKLDGSTHLNI FFAKSSEEELAGVATWPWDKEALMH LGGIVLNPSFYGMPGHTHTM IHEIGHSLGLYHVFRGISEIQSCSD PCMETEPSFETGDLCNDTNPAPKHK SCGDPGPGNDTCGFHSFFNT PYNNFMSYADDDCTDSFTPNQVARM HCYLDLVYQGWQPSRKPAPVALAPQ VLGHTTDSVTLEWFPPIDGH FFERELGSACHLCLEGRILVQYASN ASSPMPCSPSGHWSPREAEGHPDVE QPCKSSVRTWSPNSAVNPHT VPPACPEPQGCYLELEFLYPLVPES LTIWVTFVSTDWDSSGAVNDIKLLA VSGKNISLGPQNVFCDVPLT IRLWDVGEEVYGIQIYTLDEHLEID AAMLTSTADTPLCLQCKPLKYKVVR DPPLQMDVASILHLNRKFVD MDLNLGSVYQYWVITISGTEESEPS PAVTYIHGSGYCGDGIIQKDQGEQC DDMNKINGDGCSLFCRQEVS FNCIDEPSRCYFHDGDGVCEEFEQK TSIKDCGVYTPQGFLDQWASNASVS HQDQQCPGWVIIGQPAASQV CRTKVIDLSEGISQHAWYPCTISYP YSQLAQTTFWLRAYFSQPMVAAAVI VHLVTDGTYYGDQKQETISV QLLDTKDQSHDLGLHVLSCRNNPLI IPVVHDLSQPFYHSQAVRVSFSSPL VAISGVALRSFDNFDPVTLS SCQRGETYSPAEQSCVHFACEKTDC PELAVENASLNCSSSDRYHGAQCTV SCRTGYVLQIRRDDELIKSQ TGPSVTVTCTEGKWNKQVACEPVDC SIPDHHQVYAASFSCPEGTTFGSQC SFQCRHPAQLKGNNSLLTCM EDGLWSFPEALCELMCLAPPPVPNA DLQTARCRENKHKVGSFCKYKCKPG YHVPGSSRKSKKRAFKTQCT QDGSWQEGACVPVTCDPPPPKFHGL YQCTNGFQFNSECRIKCEDSDASQG LGSNVIHCRKDGTWNGSFHV CQEMQGQCSVPNELNSNLKLQCPDG YAIGSECATSCLDHNSESIILPMNV TVRDIPHWLNPTRVERVVCT AGLKWYPHPALIHCVKGCEPFMGDN YCDAINNRAFCNYDGGDCCTSTVKT KKVTPFPMSCDLQGDCACRD PQAQEHSRKDLRGYSHG SGPTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease, Secreted Protein

Gene Information

• Gene Name: PAPPA pappalysin 1 [ Homo sapiens (human) ]
• Official Symbol: PAPPA
• Synonyms: PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase
• Gene ID: 5069
• mRNA Refseq: NM_002581.5
• Protein Refseq: NP_002572.2
• MIM: 176385
• UniProt ID: Q13219