Recombinant Full Length Human PCCA Protein, C-Flag-tagged

• Cat.No.: PCCA-502HFL
• Product Overview: Recombinant Full Length Human PCCA Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 75 kDa
• AA Sequence: MAGFWVGTAPLVAAGRRGRWPPQQL MLSAALRTLKHVLYYSRQCLMVSRN LGSVGYDPNEKTFDKILVAN RGEIACRVIRTCKKMGIKTVAIHSD VDASSVHVKMADEAVCVGPAPTSKS YLNMDAIMEAIKKTRAQAVH PGYGFLSENKEFARCLAAEDVVFIG PDTHAIQAMGDKIESKLLAKKAEVN TIPGFDGVVKDAEEAVRIAR EIGYPVMIKASAGGGGKGMRIAWDD EETRDGFRLSSQEAASSFGDDRLLI EKFIDNPRHIEIQVLGDKHG NALWLNERECSIQRRNQKVVEEAPS IFLDAETRRAMGEQAVALARAVKYS SAGTVEFLVDSKKNFYFLEM NTRLQVEHPVTECITGLDLVQEMIR VAKGYPLRHKQADIRINGWAVECRV YAEDPYKSFGLPSIGRLSQY QEPLHLPGVRVDSGIQPGSDISIYY DPMISKLITYGSDRTEALKRMADAL DNYVIRGVTHNIALLREVII NSRFVKGDISTKFLSDVYPDGFKGH MLTKSEKNQLLAIASSLFVAFQLRA QHFQENSRMPVIKPDIANWE LSVKLHDKVHTVVASNNGSVFSVEV DGSKLNVTSTWNLASPLLSVSVDGT QRTVQCLSREAGGNMSIQFL GTVYKVNILTRLAAELNKFMLEKVT EDTSSVLRSPMPGVVVAVSVKPGDA VAEGQEICVIEAMKMQNSMT
  AGKTGTVKSVHCQAGDTVGEGDLLV ELETRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation

Gene Information

• Gene Name: PCCA propionyl-CoA carboxylase subunit alpha [ Homo sapiens (human) ]
• Official Symbol: PCCA
• Synonyms: PCCase alpha subunit; propanoyl-CoA:carbon dioxide ligase alpha subunit; propionyl-CoA carboxylase alpha chain, mitochondrial; propionyl-coenzyme A carboxylase, alpha polypeptide; Propionyl Coenzyme A carboxylase alpha polypeptide
• Gene ID: 5095
• mRNA Refseq: NM_000282.4
• Protein Refseq: NP_000273.2
• MIM: 232000
• UniProt ID: P05165