Recombinant Full Length Human PEPD Protein, C-Flag-tagged

• Cat.No.: PEPD-1390HFL
• Product Overview: Recombinant Full Length Human PEPD Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 54.4 kDa
• AA Sequence: MAAATGPSFWLGNETLKVPLALFAL NRQRLCERLRKNPAVQAGSIVVLQG GEETQRYCTDTGVLFRQESF FHWAFGVTEPGCYGVIDVDTGKSTL FVPRLPASHATWMGKIHSKEHFKEK YAVDDVQYVDEIASVLTSQK PSVLLTLRGVNTDSGSVCREASFDG ISKFEVNNTILHPEIVECRVFKTDM ELEVLRYTNKISSEAHREVM KAVKVGMKEYELESLFEHYCYSRGG MRHSSYTCICGSGENSAVLHYGHAG APNDRTIQNGDMCLFDMGGE YYCFASDITCSFPANGKFTADQKAV YEAVLRSSRAVMGAMKPGVWWPDMH RLADRIHLEELAHMGILSGS VDAMVQAHLGAVFMPHGLGHFLGID VHDVGGYPEGVERIDEPGLRSLRTA RHLQPGMVLTVEPGIYFIDH LLDEALADPARASFLNREVLQRFRG FGGVRIEEDVVVTDSGIELLTCVPR TVEEIEACMAGCDKAFTPFS
  GPKTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease

Gene Information

• Gene Name: PEPD peptidase D [ Homo sapiens (human) ]
• Official Symbol: PEPD
• Synonyms: PROLIDASE
• Gene ID: 5184
• mRNA Refseq: NM_000285.4
• Protein Refseq: NP_000276.2
• MIM: 613230
• UniProt ID: P12955