Recombinant Full Length Human PGF Protein, C-His-tagged

• Cat.No.: PGF-21HFL
• Product Overview: Recombinant Full Length Human PGF Protein, fused to His-tag, was expressed in HEK293T.

Specification

• Description: PIGF-2, as know as placenta growth factor, is a member of the VEGF sub-family that a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. It is ultimately associated with angiogenesis. Specifically, it plays a role in trophoblast growth and differentiation. Also it is a potential biomarker for preeclampsia, a condition in which blood vessels in the placenta are too narrow, resulting in high blood pressure. Under normal physiologic conditions, it is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal muscle. Recombinant human PIGF-2, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.
• Source: HEK293T
• Species: Human
• Tag: C-His
• Protein length: 19-170 aa
• Form: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
• Bio-activity: Measured by its binding ability in a functional ELISA with Human VEGFR1/Flt-1.
• Molecular Mass: 18.1kDa
• AA Sequence: LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
• Purity: > 90% by SDS - PAGE.
• Applications: SDS-PAGE, Bioactivity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm).
• References: Asma Khalil., et al, (2008) PLoS One. 3:e2766.
  Khurana R., et al, (2005) Circulation. 111:2828-2836.

Gene Information

• Gene Name: PGF placental growth factor [ Homo sapiens (human) ]
• Official Symbol: PGF
• Synonyms: PGF; placental growth factor; placenta growth factor isoform 1; D12S1900; PGFL; PIGF; PLGF; PlGF-2; SHGC-10760
• Gene ID: 5228
• mRNA Refseq: NM_002632.6
• Protein Refseq: NP_002623.2
• MIM: 601121
• UniProt ID: P49763