Recombinant Full Length Human PLB1 Protein, C-Flag-tagged

• Cat.No.: PLB1-1311HFL
• Product Overview: Recombinant Full Length Human PLB1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a membrane-associated phospholipase that displays lysophospholipase and phospholipase A2 activities through removal of sn-1 and sn-2 fatty acids of glycerophospholipids. In addition, it displays lipase and retinyl ester hydrolase activities. The encoded protein is highly conserved and is composed of a large, glycosylated extracellular domain composed of four tandem homologous domains, followed by a hydrophobic segment that anchors the enzyme to the membrane and a short C-terminal cytoplasmic tail. This gene has been identified as a candidate rheumatoid arthritis risk gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 162.9 kDa
• AA Sequence: MGLRPGIFLLELLLLLGQGTPQIHT SPRKSTLEGQLWPETLKNSPFPCNP NKLGVNMPSKSVHSLKPSDI KFVAAIGNLEIPPDPGTGDLEKQDW TERPQQVCMGVMTVLSDIIRYFSPS VPMPVCHTGKRVIPHDGAED LWIQAQELVRNMKENLQLDFQFDWK LINVFFSNASQCYLCPSAQQNGLAA GGVDELMGVLDYLQQEVPRA FVNLVDLSEVAEVSRQYHGTWLSPA PEPCNCSEETTRLAKVVMQWSYQEA WNSLLASSRYSEQESFTVVF QPFFYETTPSLHSEDPRLQDSTTLA WHLWNRMMEPAGEKDEPLSVKHGRP MKCPSQESPYLFSYRNSNYL TRLQKPQDKLEVREGAEIRCPDKDP SDTVPTSVHRLKPADINVIGALGDS LTAGNGAGSTPGNVLDVLTQ YRGLSWSVGGDENIGTVTTLANILR EFNPSLKGFSVGTGKETSPNAFLNQ AVAGGRAEDLPVQARRLVDL MKNDTRIHFQEDWKIITLFIGGNDL CDFCNDLVHYSPQNFTDNIGKALDI LHAEVPRAFVNLVTVLEIVN LRELYQEKKVYCPRMILRSLCPCVL KFDDNSTELATLIEFNKKFQEKTHQ LIESGRYDTREDFTVVVQPF FENVDMPKTSEGLPDNSFFAPDCFH FSSKSHSRAASALWNNMLEPVGQKT TRHKFENKINITCPNQVQPF LRTYKNSMQGHGTWLPCRDRAPSAL HPTSVHALRPADIQVVAALGDSLTA GNGIGSKPDDLPDVTTQYRG LSYSAGGDGSLENVTTLPNILREFN RNLTGYAVGTGDANDTNAFLNQAVP GAKAEDLMSQVQTLMQKMKD DHRVNFHEDWKVITVLIGGSDLCDY CTDSNLYSAANFVDHLRNALDVLHR EVPRVLVNLVDFLNPTIMRQ VFLGNPDKCPVQQASVLCNCVLTLR ENSQELARLEAFSRAYRSSMRELVG SGRYDTQEDFSVVLQPFFQN IQLPVLADGLPDTSFFAPDCIHPNQ KFHSQLARALWTNMLEPLGSKTETL DLRAEMPITCPTQNEPFLRT PRNSNYTYPIKPAIENWGSDFLCTE WKASNSVPTSVHQLRPADIKVVAAL GDSLTTAVGARPNNSSDLPT SWRGLSWSIGGDGNLETHTTLPNIL KKFNPYLLGFSTSTWEGTAGLNVAA EGARARDMPAQAWDLVERMK NSPDINLEKDWKLVTLFIGVNDLCH YCENPEAHLATEYVQHIQQALDILS EELPRAFVNVVEVMELASLY QGQGGKCAMLAAQNNCTCLRHSQSS LEKQELKKVNWNLQHGISSFSYWHQ YTQREDFAVVVQPFFQNTLT PLNERGDTDLTFFSEDCFHFSDRGH AEMAIALWNNMLEPVGRKTTSNNFT HSRAKLKCPSPESPYLYTLR
  NSRLLPDQAEEAPEVLYWAVPVAAG VGLVVGIIGTVVWRCRRGGRREDPP MSLRTVALTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane

Gene Information

• Gene Name: PLB1 phospholipase B1 [ Homo sapiens (human) ]
• Official Symbol: PLB1
• Synonyms: PLB; PLB/LIP
• Gene ID: 151056
• mRNA Refseq: NM_153021.5
• Protein Refseq: NP_694566.4
• MIM: 610179
• UniProt ID: Q6P1J6