Recombinant Full Length Human PLCG1 Protein, C-Flag-tagged

• Cat.No.: PLCG1-94HFL
• Product Overview: Recombinant Full Length Human PLCG1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 148.5 kDa
• AA Sequence: MAGAASPCANGCGPGAPSDAEVLHL CRSLEVGTVMTLFYSKKSQRPERKT FQVKLETRQITWSRGADKIE GAIDIREIKEIRPGKTSRDFDRYQE DPAFRPDQSHCFVILYGMEFRLKTL SLQATSEDEVNMWIKGLTWL MEDTLQAPTPLQIERWLRKQFYSVD RNREDRISAKDLKNMLSQVNYRVPN MRFLRERLTDLEQRSGDITY GQFAQLYRSLMYSAQKTMDLPFLEA STLRAGERPELCRVSLPEFQQFLLD YQGELWAVDRLQVQEFMLSF LRDPLREIEEPYFFLDEFVTFLFSK ENSVWNSQLDAVCPDTMNNPLSHYW ISSSHNTYLTGDQFSSESSL EAYARCLRMGCRCIELDCWDGPDGM PVIYHGHTLTTKIKFSDVLHTIKEH AFVASEYPVILSIEDHCSIA QQRNMAQYFKKVLGDTLLTKPVEIS ADGLPSPNQLKRKILIKHKKLAEGS AYEEVPTSMMYSENDISNSI KNGILYLEDPVNHEWYPHYFVLTSS KIYYSEETSSDQGNEDEEEPKEVSS STELHSNEKWFHGKLGAGRD GRHIAERLLTEYCIETGAPDGSFLV RESETFVGDYTLSFWRNGKVQHCRV HSRQDAGTPKFFLTDNLVFD SLYDLITHYQQVPLRCNEFEMRLSE PVPQTNAHESKEWYHASLTRAQAEH MLMRVPRDGAFLVRKRNEPN SYAISFRAEGKIKHCRVQQEGQTVM LGNSEFDSLVDLISYYEKHPLYRKM KLRYPINEEALEKIGTAEPD YGALYEGRNPGFYVEANPMPTFKCA VKALFDYKAQREDELTFTKSAIIQN VEKQEGGWWRGDYGGKKQLW FPSNYVEEMVNPVALEPEREHLDEN SPLGDLLRGVLDVPACQIAIRPEGK NNRLFVFSISMASVAHWSLD VAADSQEELQDWVKKIREVAQTADA RLTEGKIMERRKKIALELSELVVYC RPVPFDEEKIGTERACYRDM SSFPETKAEKYVNKAKGKKFLQYNR LQLSRIYPKGQRLDSSNYDPLPMWI CGSQLVALNFQTPDKPMQMN QALFMTGRHCGYVLQPSTMRDEAFD PFDKSSLRGLEPCAISIEVLGARHL PKNGRGIVCPFVEIEVAGAE YDSTKQKTEFVVDNGLNPVWPAKPF HFQISNPEFAFLRFVVYEEDMFSDQ NFLAQATFPVKGLKTGYRAV PLKNNYSEDLELASLLIKIDIFPAK QENGDLSPFSGTSLRERGSDASGQL FHGRAREGSFESRYQQPFED
  FRISQEHLADHFDSRERRAPRRTRV NGDNRLTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, T cell receptor signaling pathway, VEGF signaling pathway, Vibrio cholerae infection

Gene Information

• Gene Name: PLCG1 phospholipase C gamma 1 [ Homo sapiens (human) ]
• Official Symbol: PLCG1
• Synonyms: PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
• Gene ID: 5335
• mRNA Refseq: NM_002660.3
• Protein Refseq: NP_002651.2
• MIM: 172420
• UniProt ID: P19174