Recombinant Full Length Human PPAT Protein, C-Flag-tagged

• Cat.No.: PPAT-1367HFL
• Product Overview: Recombinant Full Length Human PPAT Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 57.2 kDa
• AA Sequence: MELEELGIREECGVFGCIASGEWPT QLDVPHVITLGLVGLQHRGQESAGI VTSDGSSVPTFKSHKGMGLV NHVFTEDNLKKLYVSNLGIGHTRYA TTGKCELENCQPFVVETLHGKIAVA HNGELVNAARLRKKLLRHGI GLSTSSDSEMITQLLAYTPPQEQDD TPDWVARIKNLMKEAPTAYSLLIMH RDVIYAVRDPYGNRPLCIGR LIPVSDINDKEKKTSETEGWVVSSE SCSFLSIGARYYREVLPGEIVEISR HNVQTLDIISRSEGNPVAFC IFEYVYFARPDSMFEDQMVYTVRYR CGQQLAIEAPVDADLVSTVPESATP AALAYAGKCGLPYVEVLCKN RYVGRTFIQPNMRLRQLGVAKKFGV LSDNFKGKRIVLVDDSIVRGNTISP IIKLLKESGAKEVHIRVASP PIKYPCFMGINIPTKEELIANKPEF DHLAEYLGANSVVYLSVEGLVSSVQ EGIKFKKQKEKKHDIMIQEN
  GNGLECFEKSGHCTACLTGKYPVEL EWTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism

Gene Information

• Gene Name: PPAT phosphoribosyl pyrophosphate amidotransferase [ Homo sapiens (human) ]
• Official Symbol: PPAT
• Synonyms: GPAT; PRAT; ATASE
• Gene ID: 5471
• mRNA Refseq: NM_002703.5
• Protein Refseq: NP_002694.3
• MIM: 172450
• UniProt ID: Q06203