Recombinant Full Length Human PRC1 Protein, C-Flag-tagged

• Cat.No.: PRC1-1623HFL
• Product Overview: Recombinant Full Length Human PRC1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 71.5 kDa
• AA Sequence: MRRSEVLAEESIVCLQKALNHLREI WELIGIPEDQRLQRTEVVKKHIKEL LDMMIAEEESLKERLIKSIS VCQKELNTLCSELHVEPFQEEGETT ILQLEKDLRTQVELMRKQKKERKQE LKLLQEQDQELCEILCMPHY DIDSASVPSLEELNQFRQHVTTLRE TKASRREEFVSIKRQIILCMEELDH TPDTSFERDVVCEDEDAFCL SLENIATLQKLLRQLEMQKSQNEAV CEGLRTQIRELWDRLQIPEEEREAV ATIMSGSKAKVRKALQLEVD RLEELKMQNMKKVIEAIRVELVQYW DQCFYSQEQRQAFAPFCAEDYTESL LQLHDAEIVRLKNYYEVHKE LFEGVQKWEETWRLFLEFERKASDP NRFTNRGGNLLKEEKQRAKLQKMLP KLEEELKARIELWEQEHSKA FMVNGQKFMEYVAEQWEMHRLEKER AKQERQLKNKKQTETEMLYGSAPRT PSKRRGLAPNTPGKARKLNT TTMSNATANSSIRPIFGGTVYHSPV SRLPPSGSKPVAASTCSGKKTPRTG RHGANKENLELNGSILSGGY
  PGSAPLQRNFSINSVASTYSEFAKD PSLSDSSTVGLQRELSKASKSDATS GILNSTNIQSTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: PRC1 protein regulator of cytokinesis 1 [ Homo sapiens (human) ]
• Official Symbol: PRC1
• Synonyms: ASE1
• Gene ID: 9055
• mRNA Refseq: NM_003981.4
• Protein Refseq: NP_003972.2
• MIM: 603484
• UniProt ID: O43663

Reviews

03/29/2023

It has high safety, environmental protection and no pollution.

12/02/2022

The quality is so good, it greatly saves the experimental time, very good.

10/28/2021

The preparation cost is low and cost-effective, which is very suitable for our research.

Q&As

How is PRC1 activity regulated?

The activity of PRC1 can be modulated by different regulatory mechanisms, including phosphorylation and dephosphorylation, interaction with other proteins, and small molecule regulation. These regulatory mechanisms can affect the interaction of PRC1 with microtubules and its function in cell division.

Is PRC1 involved in cell division?

PRC1 promotes chromosome segregation and cytoplasmic equalization during metaphase of cell division by interacting with microtubules. It is also involved in the process of cell plate formation.

What is the relationship of PRC1 to other proteins?

It interacts with other proteins, such as microtubule-associated proteins and cyclins. These interactions help regulate the function of PRC1 in cell division.

Does PRC1 have a role in stem cell and tissue regeneration?

PRC1 plays an important role in stem cell and tissue regeneration. It is involved in the process of stem cell self-renewal and differentiation, as well as the cell cycle regulation of tissue regeneration.

How about drug development for PRC1?

Drug development for PRC1 is ongoing. Several research teams are developing small molecule inhibitors or activators that modulate PRC1 activity for the treatment of PRC1-related diseases.

How is PRC1 expression regulated?

The expression level of PRC1 is regulated by a variety of factors, including transcription factors, epigenetic modifications, and signal transduction pathways. These factors can affect the transcription and translation process of the PRC1 gene, thereby regulating its expression level in cells.