Recombinant Full Length Human PRMT5 Protein, C-Flag-tagged

• Cat.No.: PRMT5-688HFL
• Product Overview: Recombinant Full Length Human PRMT5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 72.5 kDa
• AA Sequence: MAAMAVGGAGGSRVSSGRDLNCVPE IADTLGAVAKQGFDFLCMPVFHPRF KREFIQEPAKNRPGPQTRSD LLLSGRDWNTLIVGKLSPWIRPDSK VEKIRRNSEAAMLQELNFGAYLGLP AFLLPLNQEDNTNLARVLTN HIHTGHHSSMFWMRVPLVAPEDLRD DIIENAPTTHTEEYSGEEKTWMWWH NFRTLCDYSKRIAVALEIGA DLPSNHVIDRWLGEPIKAAILPTSI FLTNKKGFPVLSKMHQRLIFRLLKL EVQFIITGTNHHSEKEFCSY LQYLEYLSQNRPPPNAYELFAKGYE DYLQSPLQPLMDNLESQTYEVFEKD PIKYSQYQQAIYKCLLDRVP EEEKDTNVQVLMVLGAGRGPLVNAS LRAAKQADRRIKLYAVEKNPNAVVT LENWQFEEWGSQVTVVSSDM REWVAPEKADIIVSELLGSFADNEL SPECLDGAQHFLKDDGVSIPGEYTS FLAPISSSKLYNEVRACREK DRDPEAQFEMPYVVRLHNFHQLSAP QPCFTFSHPNRDPMIDNNRYCTLEF PVEVNTVLHGFAGYFETVLY QDITLSIRPETHSPGMFSWFPILFP IKQPITVREGQTICVRFWRCSNSKK VWYEWAVTAPVCSAIHNPTG
  RSYTIGLTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Stem cell - Pluripotency

Gene Information

• Gene Name: PRMT5 protein arginine methyltransferase 5 [ Homo sapiens (human) ]
• Official Symbol: PRMT5
• Synonyms: HSL7; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• Gene ID: 10419
• mRNA Refseq: NM_006109.5
• Protein Refseq: NP_006100.2
• MIM: 604045
• UniProt ID: O14744