Recombinant Full Length Human PTBP1 Protein, C-Flag-tagged

• Cat.No.: PTBP1-530HFL
• Product Overview: Recombinant Full Length Human PTBP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 59.5 kDa
• AA Sequence: MDGIVPDIAVGTKRGSDELFSTCVT NGPFIMSSNSASAANGNDSKKFKGD SRSAGVPSRVIHIRKLPIDV TEGEVISLGLPFGKVTNLLMLKGKN QAFIEMNTEEAANTMVNYYTSVTPV LRGQPIYIQFSNHKELKTDS SPNQARAQAALQAVNSVQSGNLALA ASAAAVDAGMAMAGQSPVLRIIVEN LFYPVTLDVLHQIFSKFGTV LKIITFTKNNQFQALLQYADPVSAQ HAKLSLDGQNIYNACCTLRIDFSKL TSLNVKYNNDKSRDYTRPDL PSGDSQPSLDQTMAAAFGAPGIISA SPYAGAGFPPTFAIPQAAGLSVPNV HGALAPLAIPSAAAAAAAAG RIAIPGLAGAGNSVLLVSNLNPERV TPQSLFILFGVYGDVQRVKILFNKK ENALVQMADGNQAQLAMSHL NGHKLHGKPIRITLSKHQNVQLPRE GQEDQGLTKDYGNSPLHRFKKPGSK NFQNIFPPSATLHLSNIPPS VSEEDLKVLFSSNGGVVKGFKFFQK DRKMALIQMGSVEEAVQALIDLHNH DLGENHHLRVSFSKSTITRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: PTBP1 polypyrimidine tract binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: PTBP1
• Synonyms: PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I
• Gene ID: 5725
• mRNA Refseq: NM_002819.5
• Protein Refseq: NP_002810.1
• MIM: 600693
• UniProt ID: P26599