Recombinant Full Length Human PTEN Protein, C-Flag-tagged

• Cat.No.: PTEN-114HFL
• Product Overview: Recombinant Full Length Human PTEN Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 47 kDa
• AA Sequence: MTAIIKEIVSRNKRRYQEDGFDLDL TYIYPNIIAMGFPAERLEGVYRNNI DDVVRFLDSKHKNHYKIYNL CAERHYDTAKFNCRVAQYPFEDHNP PQLELIKPFCEDLDQWLSEDDNHVA AIHCKAGKGRTGVMICAYLL HRGKFLKAQEALDFYGEVRTRDKKG VTIPSQRRYVYYYSYLLKNHLDYRP VALLFHKMMFETIPMFSGGT CNPQFVVCQLKVKIYSSNSGPTRRE DKFMYFEFPQPLPVCGDIKVEFFHK QNKMLKKDKMFHFWVNTFFI PGPEETSEKVENGSLCDQEIDSICS IERADNDKEYLVLTLTKNDLDKANK DKANRYFSPNFKVKLYFTKT
  VEEPSNPEASSSTSVTPDVSDNEPD HYRYSDTTDSDPENEPFDEDQHTQI TKVTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Endometrial cancer, Focal adhesion, Glioma, Inositol phosphate metabolism, Melanoma, p53 signaling pathway, Pathways in cancer, Phosphatidylinositol signaling system, Prostate cancer, Small cell lung cancer, Tight junction

Gene Information

• Gene Name: PTEN phosphatase and tensin homolog [ Homo sapiens (human) ]
• Official Symbol: PTEN
• Synonyms: BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
• Gene ID: 5728
• mRNA Refseq: NM_000314.8
• Protein Refseq: NP_000305.3
• MIM: 601728
• UniProt ID: P60484

Reviews

11/05/2022

Gel electrophoresis consistently displayed well-defined bands, affirming the product's purity and reliability.

01/18/2021

The insights gained from the product's application justified its cost, marking a prudent investment in our research endeavors.

12/20/2020

Comprehensive documentation streamlined the integration of the product into our experimental workflow.

Q&As

Apart from its lipid phosphatase activity, what additional function has been attributed to PTEN?

In addition to its lipid phosphatase activity, PTEN has an additional function known as a scaffolding protein that contributes to its tumor-suppressive role.

In which cellular compartment does PTEN primarily exert its lipid phosphatase function?

PTEN primarily exerts its lipid phosphatase function in the cytoplasm, where it regulates the PI3K/AKT pathway.

What is the significance of the FBXO22-mediated degradation of nuclear PTEN in promoting tumorigenesis?

The significance of FBXO22-mediated degradation of nuclear PTEN in promoting tumorigenesis involves the loss of PTEN's tumor-suppressive functions, leading to uncontrolled cell growth.

How is the expression of nuclear PTEN (核PTEN) regulated, especially in the context of tumorigenesis?

The expression of nuclear PTEN (核PTEN) is regulated, especially in the context of tumorigenesis, through mechanisms involving post-translational modifications and subcellular localization.

What differences are observed in the expression patterns of nuclear PTEN and cytoplasmic PTEN (浆PTEN) in various cancer processes?

Differences in expression patterns of nuclear PTEN and cytoplasmic PTEN in various cancer processes include alterations in nuclear PTEN levels contributing to tumorigenesis.

What is the classical function of the PTEN protein encoded by the PTEN gene?

The classical function of the PTEN protein is to regulate cell growth and prevent tumor formation by suppressing the PI3K/AKT signaling pathway.

What role does nuclear PTEN play in mediating the non-phosphatase-dependent function?

Nuclear PTEN plays a role in mediating the non-phosphatase-dependent function related to genomic stability and DNA repair.