Recombinant Full Length Human PTK2B Protein, C-Flag-tagged

• Cat.No.: PTK2B-1427HFL
• Product Overview: Recombinant Full Length Human PTK2B Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 115.7 kDa
• AA Sequence: MSGVSEPLSRVKLGTLRRPEGPAEP MVVVPVDVEKEDVRILKVCFYSNSF NPGKNFKLVKCTVQTEIREI ITSILLSGRIGPNIRLAECYGLRLK HMKSDEIHWLHPQMTVGEVQDKYEC LHVEAEWRYDLQIRYLPEDF MESLKEDRTTLLYFYQQLRNDYMQR YASKVSEGMALQLGCLELRRFFKDM PHNALDKKSNFELLEKEVGL DLFFPKQMQENLKPKQFRKMIQQTF QQYASLREEECVMKFFNTLAGFANI DQETYRCELIQGWNITVDLV IGPKGIRQLTSQDAKPTCLAEFKQI RSIRCLPLEEGQAVLQLGIEGAPQA LSIKTSSLAEAENMADLIDG YCRLQGEHQGSLIIHPRKDGEKRNS LPQIPMLNLEARRSHLSESCSIESD IYAEIPDETLRRPGGPQYGI AREDVVLNRILGEGFFGEVYEGVYT NHKGEKINVAVKTCKKDCTLDNKEK FMSEAVIMKNLDHPHIVKLI GIIEEEPTWIIMELYLYGELGHYLE RNKNSLKVLTLVLYSLQICKAMAYL ESINCVHRDIAVRNILVASP ECVKLGDFGLSRYIEDEDYYKASVT RLPIKWMSPESINFRRFTTASDVWM FAVCMWEILSFGKQPFFWLE NKDVIGVLEKGDRLPKPDLCPPVLY TLMTRCWDYDPSDRPRFTELVCSLS DVYQMEKDIAMEQERNARYR TPKILEPTAFQEPPPKPSRPKYRPP PQTNLLAPKLQFQVPEGLCASSPTL TSPMEYPSPVNSLHTPPLHR HNVFKRHSMREEDFIQPSSREEAQQ LWEAEKVKMRQILDKQQKQMVEDYQ WLRQEEKSLDPMVYMNDTSP LTPEKEVGYLEFTGPPQKPPRLGAQ SIQPTANLDRTDDLVYLNVMELVRA VLELKNELCQLPPEGYVVVV KNVGLTLRKLIGSVDDLLPSLPSSS RTEIEGTQKLLNKDLAELINKMRLA QQNAVTSLSEECKRQMLTAS
  HTLAVDAKNLLDAVDQAKVLANLAH PPAETRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity

Gene Information

• Gene Name: PTK2B protein tyrosine kinase 2 beta [ Homo sapiens (human) ]
• Official Symbol: PTK2B
• Synonyms: PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK
• Gene ID: 2185
• mRNA Refseq: NM_173174.3
• Protein Refseq: NP_775266.1
• MIM: 601212
• UniProt ID: Q14289