Recombinant Full Length Human PYGL Protein, C-Flag-tagged

• Cat.No.: PYGL-1275HFL
• Product Overview: Recombinant Full Length Human PYGL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 97 kDa
• AA Sequence: MAKPLTDQEKRRQISIRGIVGVENV AELKKSFNRHLHFTLVKDRNVATTR DYYFALAHTVRDHLVGRWIR TQQHYYDKCPKRVYYLSLEFYMGRT LQNTMINLGLQNACDEAIYQLGLDI EELEEIEEDAGLGNGGLGRL AACFLDSMATLGLAAYGYGIRYEYG IFNQKIRDGWQVEEADDWLRYGNPW EKSRPEFMLPVHFYGKVEHT NTGTKWIDTQVVLALPYDTPVPGYM NNTVNTMRLWSARAPNDFNLRDFNV GDYIQAVLDRNLAENISRVL YPNDNFFEGKELRLKQEYFVVAATL QDIIRRFKASKFGSTRGAGTVFDAF PDQVAIQLNDTHPALAIPEL MRIFVDIEKLPWSKAWELTQKTFAY TNHTVLPEALERWPVDLVEKLLPRH LEIIYEINQKHLDRIVALFP KDVDRLRRMSLIEEEGSKRINMAHL CIVGSHAVNGVAKIHSDIVKTKVFK DFSELEPDKFQNKTNGITPR RWLLLCNPGLAELIAEKIGEDYVKD LSQLTKLHSFLGDDVFLRELAKVKQ ENKLKFSQFLETEYKVKINP SSMFDVQVKRIHEYKRQLLNCLHVI TMYNRIKKDPKKLFVPRTVIIGGKA APGYHMAKMIIKLITSVADV VNNDPMVGSKLKVIFLENYRVSLAE KVIPATDLSEQISTAGTEASGTGNM KFMLNGALTIGTMDGANVEM AEEAGEENLFIFGMRIDDVAALDKK GYEAKEYYEALPELKLVIDQIDNGF FSPKQPDLFKDIINMLFYHD RFKVFADYEAYVKCQDKVSQLYMNP KAWNTMVLKNIAASGKFSSDRTIKE YAQNIWNVEPSDLKISLSNE
  SNKVNGNTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism

Gene Information

• Gene Name: PYGL glycogen phosphorylase L [ Homo sapiens (human) ]
• Official Symbol: PYGL
• Synonyms: GSD6
• Gene ID: 5836
• mRNA Refseq: NM_002863.5
• Protein Refseq: NP_002854.3
• MIM: 613741
• UniProt ID: P06737