Recombinant Full Length Human RPLP0 Protein, C-Flag-tagged

• Cat.No.: RPLP0-1633HFL
• Product Overview: Recombinant Full Length Human RPLP0 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 34.1 kDa
• AA Sequence: MPREDRATWKSNYFLKIIQLLDDYP KCFIVGADNVGSKQMQQIRMSLRGK AVVLMGKNTMMRKAIRGHLE NNPALEKLLPHIRGNVGFVFTKEDL TEIRDMLLANKVPAAARAGAIAPCE VTVPAQNTGLGPEKTSFFQA LGITTKISRGTIEILSDVQLIKTGD KVGASEATLLNMLNISPFSFGLVIQ QVFDNGSIYNPEVLDITEET LHSRFLEGVRNVASVCLQIGYPTVA SVPHSIINGYKRVLALSVETDYTFP LAEKVKAFLADPSAFVAAAP
  VAAATTAAPAAAAAPAKVEAKEESE ESDEDMGFGLFDTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Ribosome

Gene Information

• Gene Name: RPLP0 ribosomal protein lateral stalk subunit P0 [ Homo sapiens (human) ]
• Official Symbol: RPLP0
• Synonyms: P0; LP0; L10E; RPP0; PRLP0
• Gene ID: 6175
• mRNA Refseq: NM_053275.4
• Protein Refseq: NP_444505.1
• MIM: 180510
• UniProt ID: P05388