Recombinant Full Length Human SF3B2 Protein, C-Flag-tagged

• Cat.No.: SF3B2-739HFL
• Product Overview: Recombinant Full Length Human SF3B2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 100 kDa
• AA Sequence: MATEHPEPPKAELQLPPPPPPGHYG AWAAQELQAKLAEIGAPIQGNREEL VERLQSYTRQTGIVLNRPVL RGEDGDKAAPPPMSAQLPGIPMPPP PLGLPPLQPPPPPPPPPPGLGLGFP MAHPPNLGPPPPLRVGEPVA LSEEERLKLAQQQAALLMQQEERAK QQGDHSLKEHELLEQQKRAAVLLEQ ERQQEIAKMGTPVPRPPQDM GQIGVRTPLGPRVAAPVGPVGPTPT VLPMGAPVPRPRGPPPPPGDENREM DDPSVGPKIPQALEKILQLK ESRQEEMNSQQEEEEMETDARSSLG QSASETEEDTVSVSKKEKNRKRRNR KKKKKPQRVRGVSSESSGDR EKDSTRSRGSDSPAADVEIEYVTEE PEIYEPNFIFFKRIFEAFKLTDDVK KEKEKEPEKLDKLENSAAPK KKGFEEEHKDSDDDSSDDEQEKKPE APKLSKKKLRRMNRFTVAELKQLVA RPDVVEMHDVTAQDPKLLVH LKATRNSVPVPRHWCFKRKYLQGKR GIEKPPFELPDFIKRTGIQEMREAL QEKEEQKTMKSKMREKVRPK MGKIDIDYQKLHDAFFKWQTKPKLT IHGDLYYEGKEFETRLKEKKPGDLS DELRISLGMPVGPNAHKVPP PWLIAMQRYGPPPSYPNLKIPGLNS PIPESCSFGYHAGGWGKPPVDETGK PLYGDVFGTNAAEFQTKTEE EEIDRTPWGELEPSDEESSEEEEEE ESDEDKPDETGFITPADSGLITPGG FSSVPAGMETPELIELRKKK IEEAMDGSETPQLFTVLPEKRTATV GGAMMGSTHIYDMSTVMSRKGPAPE LQGVEVALAPEELELDPMAM
  TQKYEEHVREQQAQVEKEDFSDMVA EHAAKQKQKKRKAQPQDSRGGSKKY KEFKFTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Spliceosome

Gene Information

• Gene Name: SF3B2 splicing factor 3b subunit 2 [ Homo sapiens (human) ]
• Official Symbol: SF3B2
• Synonyms: CFM; Cus1; SF3b1; SAP145; SF3B145; SF3b150
• Gene ID: 10992
• mRNA Refseq: NM_006842.3
• Protein Refseq: NP_006833.2
• MIM: 605591
• UniProt ID: Q13435