Recombinant Full Length Human SF3B3 Protein, C-Flag-tagged

• Cat.No.: SF3B3-71HFL
• Product Overview: Recombinant Full Length Human SF3B3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 135.4 kDa
• AA Sequence: MFLYNLTLQRATGISFAIHGNFSGT KQQEIVVSRGKILELLRPDPNTGKV HTLLTVEVFGVIRSLMAFRL TGGTKDYIVVGSDSGRIVILEYQPS KNMFEKIHQETFGKSGCRRIVPGQF LAVDPKGRAVMISAIEKQKL VYILNRDAAARLTISSPLEAHKANT LVYHVVGVDVGFENPMFACLEMDYE EADNDPTGEAAANTQQTLTF YELDLGLNHVVRKYSEPLEEHGNFL ITVPGGSDGPSGVLICSENYITYKN FGDQPDIRCPIPRRRNDLDD PERGMIFVCSATHKTKSMFFSLAQT EQGDIFKITLETDEDMVTEIRLKYF DTVPVAAAMCVLKTGFLFVA SEFGNHYLYQIAHLGDDDEEPEFSS AMPLEEGDTFFFQPRPLKNLVLVDE LDSLSPILFCQIADLANEDT PQLYVACGRGPRSSLRVLRHGLEVS EMAVSELPGNPNAVWTVRRHIEDEF DAYIIVSFVNATLVLSIGET VEEVTDSGFLGTTPTLSCSLLGDDA LVQVYPDGIRHIRADKRVNEWKTPG KKTIVKCAVNQRQVVIALTG GELVYFEMDPSGQLNEYTERKEMSA DVVCMSLANVPPGEQRSRFLAVGLV DNTVRIISLDPSDCLQPLSM QALPAQPESLCIVEMGGTEKQDELG ERGSIGFLYLNIGLQNGVLLRTVLD PVTGDLSDTRTRYLGSRPVK LFRVRMQGQEAVLAMSSRSWLSYSY QSRFHLTPLSYETLEFASGFASEQC PEGIVAISTNTLRILALEKL GAVFNQVAFPLQYTPRKFVIHPESN NLIIIETDHNAYTEATKAQRKQQMA EEMVEAAGEDERELAAEMAA AFLNENLPESIFGAPKAGNGQWASV IRVMNPIQGNTLDLVQLEQNEAAFS VAVCRFSNTGEDWYVLVGVA KDLILNPRSVAGGFVYTYKLVNNGE KLEFLHKTPVEEVPAAIAPFQGRVL IGVGKLLRVYDLGKKKLLRK CENKHIANYISGIQTIGHRVIVSDV QESFIWVRYKRNENQLIIFADDTYP RWVTTASLLDYDTVAGADKF GNICVVRLPPNTNDEVDEDPTGNKA LWDRGLLNGASQKAEVIMNYHVGET VLSLQKTTLIPGGSESLVYT TLSGGIGILVPFTSHEDHDFFQHVE MHLRSEHPPLCGRDHLSFRSYYFPV KNVIDGDLCEQFNSMEPNKQ
  KNVSEELDRTPPEVSKKLEDIRTRY AFTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Spliceosome

Gene Information

• Gene Name: SF3B3 splicing factor 3b subunit 3 [ Homo sapiens (human) ]
• Official Symbol: SF3B3
• Synonyms: RSE1; SAP130; SF3b130; STAF130
• Gene ID: 23450
• mRNA Refseq: NM_012426.5
• Protein Refseq: NP_036558.3
• MIM: 605592
• UniProt ID: Q15393