Recombinant Full Length Human SIAE Protein, C-Flag-tagged

• Cat.No.: SIAE-1472HFL
• Product Overview: Recombinant Full Length Human SIAE Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme which removes 9-O-acetylation modifications from sialic acids. Mutations in this gene are associated with susceptibility to autoimmune disease 6. Multiple transcript variants encoding different isoforms, found either in the cytosol or in the lysosome, have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 58.1 kDa
• AA Sequence: MVAPGLVLGLVLPLILWADRSAGIG FRFASYINNDMVLQKEPAGAVIWGF GTPGATVTVTLRQGQETIMK KVTSVKAHSDTWMVVLDPMKPGGPF EVMAQQTLEKINFTLRVHDVLFGDV WLCSGQSNMQMTVLQIFNAT RELSNTAAYQSVRILSVSPIQAEQE LEDLVAVDLQWSKPTSENLGHGYFK YMSAVCWLFGRHLYDTLQYP IGLIASSWGGTPIEAWSSGRSLKAC GVPKQGSIPYDSVTGPSKHSVLWNA MIHPLCNMTLKGVVWYQGES NINYNTDLYNCTFPALIEDWRETFH RGSQGQTERFFPFGLVQLSSDLSKK SSDDGFPQIRWHQTADFGYV PNPKMPNTFMAVAMDLCDRDSPFGS IHPRDKQTVAYRLHLGARALAYGEK NLTFEGPLPEKIELLAHKGL LNLTYYQQIQVQKKDNKIFEISCCS DHRCKWLPASMNTVSTQSLTLAIDS CHGTVVALRYAWTTWPCEYK
  QCPLYHPSSALPAPPFIAFITDQGP GHQSNVAKTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: SIAE sialic acid acetylesterase [ Homo sapiens (human) ]
• Official Symbol: SIAE
• Synonyms: LSE; AIS6; CSEC; YSG2; CSE-C
• Gene ID: 54414
• mRNA Refseq: NM_170601.5
• Protein Refseq: NP_733746.1
• MIM: 610079
• UniProt ID: Q9HAT2