Recombinant Full Length Human SUCLA2 Protein, C-Flag-tagged

• Cat.No.: SUCLA2-1717HFL
• Product Overview: Recombinant Full Length Human SUCLA2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 44.5 kDa
• AA Sequence: MAASMFYGRLVAVATLRNHRPRTAQ RAAAQVLGSSGLFNNHGLQVQQQQQ RNLSLHEYMSMELLQEAGVS VPKGYVAKSPDEAYAIAKKLGSKDV VIKAQVLAGGRGKGTFESGLKGGVK IVFSPEEAKAVSSQMIGKKL FTKQTGEKGRICNQVLVCERKYPRR EYYFAITMERSFQGPVLIGSSHGGV NIEDVAAESPEAIIKEPIDI EEGIKKEQALQLAQKMGFPPNIVES AAENMVKLYSLFLKYDATMIEINPM VEDSDGAVLCMDAKINFDSN SAYRQKKIFDLQDWTQEDERDKDAA KANLNYIGLDGNIGCLVNGAGLAMA TMDIIKLHGGTPANFLDVGG GATVHQVTEAFKLITSDKKVLAILV NIFGGIMRCDVIAQGIVMAVKDLEI KIPVVVRLQGTRVDDAKALI
  ADSGLKILACDDLDEAARMVVKLSE IVTLAKQAHVDVKFQLPITRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Propanoate metabolism

Gene Information

• Gene Name: SUCLA2 succinate-CoA ligase ADP-forming subunit beta [ Homo sapiens (human) ]
• Official Symbol: SUCLA2
• Synonyms: A-SCS; A-BETA; MTDPS5; LINC00444; SCS-betaA
• Gene ID: 8803
• mRNA Refseq: NM_003850.3
• Protein Refseq: NP_003841.1
• MIM: 603921
• UniProt ID: Q9P2R7