Recombinant Full Length Human TBC1D5 Protein, C-Flag-tagged

• Cat.No.: TBC1D5-1217HFL
• Product Overview: Recombinant Full Length Human TBC1D5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables AP-2 adaptor complex binding activity and retromer complex binding activity. Involved in several processes, including macroautophagy; positive regulation of receptor internalization; and retrograde transport, endosome to Golgi. Located in Golgi apparatus; autophagosome; and endosome membrane. Part of retromer complex. Colocalizes with AP-2 adaptor complex and Atg1/ULK1 kinase complex.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 88.8 kDa
• AA Sequence: MYHSLSETRHPLQPEEQEVGIDPLS SYSNKSGGDSNKNGRRTSSTLDSEG TFNSYRKEWEELFVNNNYLA TIRQKGINGQLRSSRFRSICWKLFL CVLPQDKSQWISRIEELRAWYSNIK EIHITNPRKVVGQQDLMINN PLSQDEGSLWNKFFQDKELRSMIEQ DVKRTFPEMQFFQQENVRKILTDVL FCYARENEQLLYKQGMHELL APIVFVLHCDHQAFLHASESAQPSE EMKTVLNPEYLEHDAYAVFSQLMET AEPWFSTFEHDGQKGKETLM TPIPFARPQDLGPTIAIVTKVNQIQ DHLLKKHDIELYMHLNRLEIAPQIY GLRWVRLLFGREFPLQDLLV VWDALFADGLSLGLVDYIFVAMLLY IRDALISSNYQTCLGLLMHYPFIGD VHSLILKALFLRDPKRNPRP VTYQFHPNLDYYKARGADLMNKSRT NAKGAPLNINKVSNSLINFGRKLIS PAMAPGSAGGPVPGGNSSSS SSVVIPTRTSAEAPSHHLQQQQQQQ RLMKSESMPVQLNKGLSSKNISSSP SVESLPGGREFTGSPPSSAT KKDSFFSNISRSRSHSKTMGRKESE EELEAQISFLQGQLNDLDAMCKYCA KVMDTHLVNIQDVILQENLE KEDQILVSLAGLKQIKDILKGSLRF NQSQLEAEENEQITIADNHYCSSGQ GQGRGQGQSVQMSGAIKQAS SETPGCTDRGNSDDFILISKDDDGS SARGSFSGQAQPLRTLRSTSGKSQA PVCSPLVFSDPLMGPASASS
  SNPSSSPDDDSSKDSGFTIVSPLDI TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: TBC1D5 TBC1 domain family member 5 [ Homo sapiens (human) ]
• Official Symbol: TBC1D5
• Synonyms: KIAA0210
• Gene ID: 9779
• mRNA Refseq: NM_014744.2
• Protein Refseq: NP_055559.1
• MIM: 615740
• UniProt ID: Q92609