Recombinant Full Length Human TCF4 Protein, C-Flag-tagged

• Cat.No.: TCF4-742HFL
• Product Overview: Recombinant Full Length Human TCF4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 71.6 kDa
• AA Sequence: MHHQQRMAALGTDKELSDLLDFSAM FSPPVSSGKNGPTSLASGHFTGSNV EDRSSSGSWGNGGHPSPSRN YGDGTPYDHMTSRDLGSHDNLSPPF VNSRIQSKTERGSYSSYGRESNLQG CHQQSLLGGDMDMGNPGTLS PTKPGSQYYQYSSNNPRRRPLHSSA MEVQTKKVRKVPPGLPSSVYAPSAS TADYNRDSPGYPSSKPATST FPSSFFMQDGHHSSDPWSSSSGMNQ PGYAGMLGNSSHIPQSSSYCSLHPH ERLSYPSHSSADINSSLPPM STFHRSGTNHYSTSSCTPPANGTDS IMANRGSGAAGSSQTGDALGKALAS IYSPDHTNNSFSSNPSTPVG SPPSLSAGTAVWSRNGGQASSSPNY EGPLHSLQSRIEDRLERLDDAIHVL RNHAVGPSTAMPGGHGDMHG IIGPSHNGAMGGLGSGYGTGLLSAN RHSLMVGTHREDGVALRGSHSLLPN QVPVPQLPVQSATSPDLNPP QDPYRGMPPGLQGQSVSSGSSEIKS DDEGDENLQDTKSSEDKKLDDDKKD IKSITRSRSSNNDDEDLTPE QKAEREKERRMANNARERLRVRDIN EAFKELGRMVQLHLKSDKPQTKLLI LHQAVAVILSLEQQVRERNL
  NPKAACLKRREEEKVSSEPPPLSLA GPHPGMGDASNHMGQMTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors

Gene Information

• Gene Name: TCF4 transcription factor 4 [ Homo sapiens (human) ]
• Official Symbol: TCF4
• Synonyms: E2-2; ITF2; PTHS; SEF2; CDG2T; FECD3; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19
• Gene ID: 6925
• mRNA Refseq: NM_001083962.2
• Protein Refseq: NP_001077431.1
• MIM: 602272
• UniProt ID: P15884