Recombinant Full Length Human TJP2 Protein, C-Flag-tagged

• Cat.No.: TJP2-2188HFL
• Product Overview: Recombinant Full Length Human TJP2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 133.8 kDa
• AA Sequence: MPVRGDRGFPPRRELSGWLRAPGME ELIWEQYTVTLQKDSKRGFGIAVSG GRDNPHFENGETSIVISDVL PGGPADGLLQENDRVVMVNGTPMED VLHSFAVQQLRKSGKVAAIVVKRPR KVQVAALQASPPLDQDDRAF EVMDEFDGRSFRSGYSERSRLNSHG GRSRSWEDSPERGRPHERARSRERD LSRDRSRGRSLERGLDQDHA RTRDRSRGRSLERGLDHDFGPSRDR DRDRSRGRSIDQDYERAYHRAYDPD YERAYSPEYRRGARHDARSR GPRSRSREHPHSRSPSPEPRGRPGP IGVLLMKSRANEEYGLRLGSQIFVK EMTRTGLATKDGNLHEGDII LKINGTVTENMSLTDARKLIEKSRG KLQLVVLRDSQQTLINIPSLNDSDS EIEDISEIESNRSFSPEERR HQYSDYDYHSSSEKLKERPSSREDT PSRLSRMGATPTPFKSTGDIAGTVV PETNKEPRYQEDPPAPQPKA APRTFLRPSPEDEAIYGPNTKMVRF KKGDSVGLRLAGGNDVGIFVAGIQE GTSAEQEGLQEGDQILKVNT QDFRGLVREDAVLYLLEIPKGEMVT ILAQSRADVYRDILACGRGDSFFIR SHFECEKETPQSLAFTRGEV FRVVDTLYDGKLGNWLAVRIGNELE KGLIPNKSRAEQMASVQNAQRDNAG DRADFWRMRGQRSGVKKNLR KSREDLTAVVSVSTKFPAYERVLLR EAGFKRPVVLFGPIADIAMEKLANE LPDWFQTAKTEPKDAGSEKS TGVVRLNTVRQIIEQDKHALLDVTP KAVDLLNYTQWFPIVIFFNPDSRQG VKTMRQRLNPTSNKSSRKLF DQANKLKKTCAHLFTATINLNSAND SWFGSLKDTIQHQQGEAVWVSEGKM EGMDDDPEDRMSYLTAMGAD YLSCDSRLISDFEDTDGEGGAYTDN ELDEPAEEPLVSSITRSSEPVQHEE SIRKPSPEPRAQMRRAASSD QLRDNSPPPAFKPEPPKAKTQNKEE SYDFSKSYEYKSNPSAVAGNETPGA STKGYPPPVAAKPTFGRSIL KPSTPIPPQEGEEVGESSEEQDNAP KSVLGKVKIFEKMDHKARLQRMQEL QEAQNARIEIAQKHPDIYAV PIKTHKPDPGTPQHTSSRPPEPQKA PSRPYQDTRGSYGSDAEEEEYRQQL SEHSKRGYYGQSARYRDTEL TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Tight junction, Vibrio cholerae infection

Gene Information

• Gene Name: TJP2 tight junction protein 2 [ Homo sapiens (human) ]
• Official Symbol: TJP2
• Synonyms: ZO2; X104; FHCA1; PFIC4; DFNA51; DUP9q21.11; C9DUPq21.11
• Gene ID: 9414
• mRNA Refseq: NM_004817.4
• Protein Refseq: NP_004808.2
• MIM: 607709
• UniProt ID: Q9UDY2