Recombinant Full Length Human TLK1 Protein, C-Flag-tagged

• Cat.No.: TLK1-1453HFL
• Product Overview: Recombinant Full Length Human TLK1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 86.5 kDa
• AA Sequence: MSVQSSSGSLEGPPSWSQLSTSPTP GSAAAARSLLNHTPPSGRPREGAMD ELHSLDPRRQELLEARFTGV ASGSTGSTGSCSVGAKASTNNESSN HSFGSLGSLSDKESETPEKKQSESS RGRKRKAENQNESSQGKSIG GRGHKISDYFEYQGGNGSSPVRGIP PAIRSPQNSHSHSTPSSSVRPNSPS PTALAFGDHPIVQPKQLSFK IIQTDLTMLKLAALESNKIQDLEKK EGRIDDLLRANCDLRRQIDEQQKLL EKYKERLNKCISMSKKLLIE KSTQEKLSSREKSMQDRLRLGHFTT VRHGASFTEQWTDGFAFQNLVKQQE WVNQQREDIERQRKLLAKRK PPTANNSQAPSTNSEPKQRKNKAVN GAENDPFVRPNLPQLLTLAEYHEQE EIFKLRLGHLKKEEAEIQAE LERLERVRNLHIRELKRINNEDNSQ FKDHPTLNERYLLLHLLGRGGFSEV YKAFDLYEQRYAAVKIHQLN KSWRDEKKENYHKHACREYRIHKEL DHPRIVKLYDYFSLDTDTFCTVLEY CEGNDLDFYLKQHKLMSEKE ARSIVMQIVNALRYLNEIKPPIIHY DLKPGNILLVDGTACGEIKITDFGL SKIMDDDSYGVDGMDLTSQG AGTYWYLPPECFVVGKEPPKISNKV DVWSVGVIFFQCLYGRKPFGHNQSQ QDILQENTILKATEVQFPVK PVVSSEAKAFIRRCLAYRKEDRFDV HQLANDPYLLPHMRRSNSSGNLHMA GLTASPTPPSSSIITYSGPTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase

Gene Information

• Gene Name: TLK1 tousled like kinase 1 [ Homo sapiens (human) ]
• Official Symbol: TLK1
• Synonyms: PKU-beta
• Gene ID: 9874
• mRNA Refseq: NM_012290.5
• Protein Refseq: NP_036422.3
• MIM: 608438
• UniProt ID: Q9UKI8