Recombinant Full Length Human TNFSF9 Protein, C-Flag-tagged

• Cat.No.: TNFSF9-1822HFL
• Product Overview: Recombinant Full Length Human TNFSF9 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 26.4 kDa
• AA Sequence: MEYASDASLDPEAPWPPAPRARACR VLPWALVAGLLLLLLLAAACAVFLA CPWAVSGARASPGSAASPRL REGPELSPDDPAGLLDLRQGMFAQL VAQNVLLIDGPLSWYSDPGLAGVSL TGGLSYKEDTKELVVAKAGV YYVFFQLELRRVVAGEGSGSVSLAL HLQPLRSAAGAAALALTVDLPPASS EARNSAFGFQGRLLHLSAGQ RLGVHLHTEARARHAWQLTQGATVL GLFRVTPEIPAGLPSPRSE TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction

Gene Information

• Gene Name: TNFSF9 TNF superfamily member 9 [ Homo sapiens (human) ]
• Official Symbol: TNFSF9
• Synonyms: CD137L; TNLG5A; 4-1BB-L
• Gene ID: 8744
• mRNA Refseq: NM_003811.4
• Protein Refseq: NP_003802.1
• MIM: 606182
• UniProt ID: P41273