Recombinant Full Length Human TNNI1 Protein, C-Flag-tagged

• Cat.No.: TNNI1-1679HFL
• Product Overview: Recombinant Full Length Human TNNI1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 21.5 kDa
• AA Sequence: MPEVERKPKITASRKLLLKSLMLAK AKECWEQEHEEREAEKVRYLAERIP TLQTRGLSLSALQDLCRELH AKVEVVDEERYDIEAKCLHNTREIK DLKLKVMDLRGKFKRPPLRRVRVSA DAMLRALLGSKHKVSMDLRA
  NLKSVKKEDTEKERPVEVGDWRKNV EAMSGMEGRKKMFDAAKSPTSQTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: TNNI1 troponin I1, slow skeletal type [ Homo sapiens (human) ]
• Official Symbol: TNNI1
• Synonyms: TNN1; SSTNI
• Gene ID: 7135
• mRNA Refseq: NM_003281.4
• Protein Refseq: NP_003272.3
• MIM: 191042
• UniProt ID: P19237