Recombinant Full Length Human TRIM28 Protein, C-Flag-tagged

• Cat.No.: TRIM28-218HFL
• Product Overview: Recombinant Full Length Human TRIM28 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 88.4 kDa
• AA Sequence: MAASAAAASAAAASAASGSPGPGEG SAGGEKRSTAPSAAASASASAAASS PAGGGAEALELLEHCGVCRE RLRPEREPRLLPCLHSACSACLGPA APAAANSSGDGGAAGDGTVVDCPVC KQQCFSKDIVENYFMRDSGS KAATDAQDANQCCTSCEDNAPATSY CVECSEPLCETCVEAHQRVKYTKDH TVRSTGPAKSRDGERTVYCN VHKHEPLVLFCESCDTLTCRDCQLN AHKDHQYQFLEDAVRNQRKLLASLV KRLGDKHATLQKSTKEVRSS IRQVSDVQKRVQVDVKMAILQIMKE LNKRGRVLVNDAQKVTEGQQERLER QHWTMTKIQKHQEHILRFAS WALESDNNTALLLSKKLIYFQLHRA LKMIVDPVEPHGEMKFQWDLNAWTK SAEAFGKIVAERPGTNSTGP APMAPPRAPGPLSKQGSGSSQPMEV QEGYGFGSGDDPYSSAEPHVSGVKR SRSGEGEVSGLMRKVPRVSL ERLDLDLTADSQPPVFKVFPGSTTE DYNLIVIERGAAAAATGQPGTAPAG TPGAPPLAGMAIVKEEETEA AIGAPPTATEGPETKPVLMALAEGP GAEGPRLASPSGSTSSGLEVVAPEG TSAPGGGPGTLDDSATICRV CQKPGDLVMCNQCEFCFHLDCHLPA LQDVPGEEWSCSLCHVLPDLKEEDG SLSLDGADSTGVVAKLSPAN QRKCERVLLALFCHEPCRPLHQLAT DSTFSLDQPGGTLDLTLIRARLQEK LSPPYSSPQEFAQDVGRMFK QFNKLTEDKADVQSIIGLQRFFETR MNEAFGDTKFSAVLVEPPPMSLPGA GLSSQELSGGPGDGPTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Protein Kinase, Stem cell - Pluripotency, Transcription Factors

Gene Information

• Gene Name: TRIM28 tripartite motif containing 28 [ Homo sapiens (human) ]
• Official Symbol: TRIM28
• Synonyms: KAP1; TF1B; RNF96; TIF1B; PPP1R157; TIF1beta
• Gene ID: 10155
• mRNA Refseq: NM_005762.3
• Protein Refseq: NP_005753.1
• MIM: 601742
• UniProt ID: Q13263