Recombinant Full Length Human TRIM32 Protein, C-Flag-tagged

• Cat.No.: TRIM32-839HFL
• Product Overview: Recombinant Full Length Human TRIM32 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 71.8 kDa
• AA Sequence: MAAAAASHLNLDALREVLECPICME SFTEEQLRPKLLHCGHTICRQCLEK LLASSINGVRCPFCSKITRI TSLTQLTDNLTVLKIIDTAGLSEAV GLLMCRSCGRRLPRQFCRSCGLVLC EPCREADHQPPGHCTLPVKE AAEERRRDFGEKLTRLRELMGELQR RKAALEGVSKDLQARYKAVLQEYGH EERRVQDELARSRKFFTGSL AEVEKSNSQVVEEQSYLLNIAEVQA VSRCDYFLAKIKQADVALLEETADE EEPELTASLPRELTLQDVEL LKVGHVGPLQIGQAVKKPRTVNVED SWAMEATASAASTSVTFREMDMSPE EVVASPRASPAKQRGPEAAS NIQQCLFLKKMGAKGSTPGMFNLPV SLYVTSQGEVLVADRGNYRIQVFTR KGFLKEIRRSPSGIDSFVLS FLGADLPNLTPLSVAMNCQGLIGVT DSYDNSLKVYTLDGHCVACHRSQLS KPWGITALPSGQFVVTDVEG GKLWCFTVDRGSGVVKYSCLCSAVR PKFVTCDAEGTVYFTQGLGLNLENR QNEHHLEGGFSIGSVGPDGQ LGRQISHFFSENEDFRCIAGMCVDA RGDLIVADSSRKEILHFPKGGGYSV LIREGLTCPVGIALTPKGQL
  LVLDCWDHCIKIYSYHLRRYSTPTR TRPLEQKLISEEDLAANDILDYKDD DDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis

Gene Information

• Gene Name: TRIM32 tripartite motif containing 32 [ Homo sapiens (human) ]
• Official Symbol: TRIM32
• Synonyms: HT2A; BBS11; TATIP; LGMD2H; LGMDR8
• Gene ID: 22954
• mRNA Refseq: NM_012210.4
• Protein Refseq: NP_036342.2
• MIM: 602290
• UniProt ID: Q13049