Recombinant Full Length Human TYR Protein, C-Flag-tagged

• Cat.No.: TYR-1016HFL
• Product Overview: Recombinant Full Length Human TYR Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 58.3 kDa
• AA Sequence: MLLAVLYCLLWSFQTSAGHFPRACV SSKNLMEKECCPPWSGDRSPCGQLS GRGSCQNILLSNAPLGPQFP FTGVDDRESWPSVFYNRTCQCSGNF MGFNCGNCKFGFWGPNCTERRLLVR RNIFDLSAPEKDKFFAYLTL AKHTISSDYVIPIGTYGQMKNGSTP MFNDINIYDLFVWMHYYVSMDALLG GSEIWRDIDFAHEAPAFLPW HRLFLLRWEQEIQKLTGDENFTIPY WDWRDAEKCDICTDEYMGGQHPTNP NLLSPASFFSSWQIVCSRLE EYNSHQSLCNGTPEGPLRRNPGNHD KSRTPRLPSSADVEFCLSLTQYESG SMDKAANFSFRNTLEGFASP LTGIADASQSSMHNALHIYMNGTMS QVQGSANDPIFLLHHAFVDSIFEQW LRRHRPLQEVYPEANAPIGH NRESYMVPFIPLYRNGDFFISSKDL GYDYSYLQDSDPDSFQDYIKSYLEQ ASRIWSWLLGAAMVGAVLTA
  LLAGLVSLLCRHKRKQLPEEKQPLL MEKEDYHSLYQSHLTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane
• Protein Pathways: Melanogenesis, Metabolic pathways, Riboflavin metabolism, Tyrosine metabolism

Gene Information

• Gene Name: TYR tyrosinase [ Homo sapiens (human) ]
• Official Symbol: TYR
• Synonyms: ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
• Gene ID: 7299
• mRNA Refseq: NM_000372.5
• Protein Refseq: NP_000363.1
• MIM: 606933
• UniProt ID: P14679