Recombinant Full Length Human USP7 Protein, C-Flag-tagged

• Cat.No.: USP7-807HFL
• Product Overview: Recombinant Full Length Human USP7 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 128.1 kDa
• AA Sequence: MNHQQQQQQQKAGEQQLSEPEDMEM EAGDTDDPPRITQNPVINGNVALSD GHNTAEEDMEDDTSWRSEAT FQFTVERFSRLSESVLSPPCFVRNL PWKIMVMPRFYPDRPHQKSVGFFLQ CNAESDSTSWSCHAQAVLKI INYRDDEKSFSRRISHLFFHKENDW GFSNFMAWSEVTDPEKGFIDDDKVT FEVFVQADAPHGVAWDSKKH TGYVGLKNQGATCYMNSLLQTLFFT NQLRKAVYMMPTEGDDSSKSVPLAL QRVFYELQHSDKPVGTKKLT KSFGWETLDSFMQHDVQELCRVLLD NVENKMKGTCVEGTIPKLFRGKMVS YIQCKEVDYRSDRREDYYDI QLSIKGKKNIFESFVDYVAVEQLDG DNKYDAGEHGLQEAEKGVKFLTLPP VLHLQLMRFMYDPQTDQNIK INDRFEFPEQLPLDEFLQKTDPKDP ANYILHAVLVHSGDNHGGHYVVYLN PKGDGKWCKFDDDVVSRCTK EEAIEHNYGGHDDDLSVRHCTNAYM LVYIRESKLSEVLQAVTDHDIPQQL VERLQEEKRIEAQKRKERQE AHLYMQVQIVAEDQFCGHQGNDMYD EEKVKYTVFKVLKNSSLAEFVQSLS QTMGFPQDQIRLWPMQARSN GTKRPAMLDNEADGNKTMIELSDNE NPWTIFLETVDPELAASGATLPKFD KDHDVMLFLKMYDPKTRSLN YCGHIYTPISCKIRDLLPVMCDRAG FIQDTSLILYEEVKPNLTERIQDYD VSLDKALDELMDGDIIVFQK DDPENDNSELPTAKEYFRDLYHRVD VIFCDKTIPNDPGFVVTLSNRMNYF QVAKTVAQRLNTDPMLLQFF KSQGYRDGPGNPLRHNYEGTLRDLL QFFKPRQPKKLYYQQLKMKITDFEN RRSFKCIWLNSQFREEEITL YPDKHGCVRDLLEECKKAVELGEKA SGKLRLLEIVSYKIIGVHQEDELLE CLSPATSRTFRIEEIPLDQV DIDKENEMLVTVAHFHKEVFGTFGI PFLLRIHQGEHFREVMKRIQSLLDI QEKEFEKFKFAIVMMGRHQY
  INEDEYEVNLKDFEPQPGNMSHPRP WLGLDHFNKAPKRSRYTYLEKAIKI HNTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease

Gene Information

• Gene Name: USP7 ubiquitin specific peptidase 7 [ Homo sapiens (human) ]
• Official Symbol: USP7
• Synonyms: TEF1; HAUSP; HAFOUS
• Gene ID: 7874
• mRNA Refseq: NM_003470.3
• Protein Refseq: NP_003461.2
• MIM: 602519
• UniProt ID: Q93009