Recombinant Full Length Human VAC14 Protein, C-Flag-tagged

• Cat.No.: VAC14-1514HFL
• Product Overview: Recombinant Full Length Human VAC14 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a scaffold protein that is a component of the PIKfyve protein kinase complex. This complex is responsible for the synthesis of phosphatidylinositol 3,5-bisphosphate, an important component of cellular membranes, from phosphatidylinositol 3-phosphate. Mice lacking a functional copy of this gene exhibit severe neurodegeneration. Mutations in the human gene have been identified in patients with a childhood onset progressive neurological disorder characterized by impaired movement, dystonia, and striatal abnormalities.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 87.8 kDa
• AA Sequence: MNPEKDFAPLTPNIVRALNDKLYEK RKVAALEIEKLVREFVAQNNTVQIK HVIQTLSQEFALSQHPHSRK GGLIGLAACSIALGKDSGLYLKELI EPVLTCFNDADSRLRYYACEALYNI VKVARGAVLPHFNVLFDGLS KLAADPDPNVKSGSELLDRLLKDIV TESNKFDLVSFIPLLRERIYSNNQY ARQFIISWILVLESVPDINL LDYLPEILDGLFQILGDNGKEIRKM CEVVLGEFLKEIKKNPSSVKFAEMA NILVIHCQTTDDLIQLTAMC WMREFIQLAGRVMLPYSSGILTAVL PCLAYDDRKKSIKEVANVCNQSLMK LVTPEDDELDELRPGQRQAE PTPDDALPKQEGTASGGPDGSCDSS FSSGISVFTAASTERAPVTLHLDGI VQVLNCHLSDTAIGMMTRIA VLKWLYHLYIKTPRKMFRHTDSLFP ILLQTLSDESDEVILKDLEVLAEIA SSPAGQTDDPGPLDGPDLQA SHSELQVPTPGRAGLLNTSGTKGLE CSPSTPTMNSYFYKFMINLLKRFSS ERKLLEVRGPFIIRQLCLLL NAENIFHSMADILLREEDLKFASTM VHALNTILLTSTELFQLRNQLKDLK TLESQNLFCCLYRSWCHNPV TTVSLCFLTQNYRHAYDLIQKFGDL EVTVDFLAEVDKLVQLIECPIFTYL RLQLLDVKNNPYLIKALYGL LMLLPQSSAFQLLSHRLQCVPNPEL LQTEDSLKAAPKSQKADSPSIDYAE LLQHFEKVQNKHLEVRHQRS
  GRGDHLDRRVVLTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: VAC14 VAC14 component of PIKFYVE complex [ Homo sapiens (human) ]
• Official Symbol: VAC14
• Synonyms: TRX; TAX1BP2; ArPIKfyve
• Gene ID: 55697
• mRNA Refseq: NM_018052.5
• Protein Refseq: NP_060522.3
• MIM: 604632
• UniProt ID: Q08AM6