Recombinant Full Length Milnesium Tardigradum Aquaporin-6(Aqp6) Protein, Tag-Free

• Cat.No.: RFL9235MF
• Product Overview: Recombinant Full Length Milnesium tardigradum Aquaporin-6(AQP6) Protein (G5CTG3) (1-285aa) was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Milnesium tardigradum (Water bear) (Tardigrade)
• Tag: Tag-Free
• Form: Lyophilized powder
• Protein length: Full Length (1-285)
• AA Sequence: MAFMDKSEVRQRLQKVLQQCAKAPT WSPKEEVRQSIFWKSIRAELIGSLV LMVFSCSRDSIYGPVSYGCTYAILS YCFKSISAHFNPVITIAALLLRSIT PFRCISLVLAQTLGTLSGASVCYYG LSNETETGSAPISPVLNVSPAKGFG YEFFGTFVIILTMSSYLDCNDYVSE SGDSNLLPLIFGLSVGLSSGMARQA TGGFLNPMRAFSLALFELNHWSNHY IYWIGPIFGCLLAVFTFDYTRPIIP NRDSNNRINFPTFNKNNKYEVEMQP ETEITLATLA Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: SDS-PAGE
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: AQP6
• Synonyms: AQP6Aquaporin-6; AQP-6
• UniProt ID: G5CTG3

Gene Information

• Gene Name: AQP6
• Synonyms: AQP6Aquaporin-6; AQP-6
• UniProt ID: G5CTG3

Reviews

01/17/2023

Its unique characteristics contribute to obtaining clear and detailed structural information, enabling researchers to unravel the intricate complexities of protein interactions and conformational changes.

04/23/2022

This protein exhibits outstanding performance in ELISA, providing accurate and reliable results in detecting and quantifying specific antigens.

10/28/2021

Its reliable and accurate performance ensures dependable results, contributing to the advancement of scientific knowledge in various disciplines.

10/01/2021

Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.

04/19/2020

By relying on the AQP6 protein and the manufacturer's exceptional technical support, researchers can confidently pursue their experimental goals while knowing they have access to reliable resources and expert guidance.

10/03/2019

Its versatility in various biological applications, along with its compatibility with different techniques, strongly recommend it as a preferred reagent for researchers in the field.

03/24/2019

Its stability and compatibility with electron microscopy techniques make it an excellent choice for studying protein structures at high resolution.

08/15/2018

Its high sensitivity ensures the detection of even low abundance targets, making it a valuable tool in immunoassays.

Q&As

Are there any pharmacological agents or drugs that specifically target AQP6?

Currently, there are no specific drugs or pharmacological agents available that specifically target AQP6. However, research is ongoing to develop compounds that can modulate the activity of aquaporin channels, including AQP6, for potential therapeutic purposes. These efforts aim to find compounds that can either enhance or inhibit AQP6 function, depending on the desired clinical outcome.

Are there any pharmacological agents or drugs that specifically target AQP6?

Currently, there are no specific drugs or pharmacological agents available that specifically target AQP6. However, research is ongoing to develop compounds that can modulate the activity of aquaporin channels, including AQP6, for potential therapeutic purposes. These efforts aim to find compounds that can either enhance or inhibit AQP6 function, depending on the desired clinical outcome.

Can genetic mutations in AQP6 lead to any disorders or diseases?

Genetic mutations in AQP6 have been associated with a rare disorder called recessive distal renal tubular acidosis (dRTA). dRTA is characterized by impaired acid secretion in the kidneys, leading to metabolic acidosis. However, more research is needed to fully understand the relationship between AQP6 mutations and the development of dRTA.

How is the expression of AQP6 regulated in the kidney?

The expression of AQP6 in the kidney is regulated by several factors. One important regulator is the hormone vasopressin, also known as antidiuretic hormone (ADH). Vasopressin acts on the renal collecting ducts and upregulates the transcription and expression of AQP2, which is closely related to AQP6. AQP2 and AQP6 share some regulatory mechanisms, and studies have shown that vasopressin can also influence AQP6 expression. Additionally, other factors such as pH levels, electrolyte balance, and hormonal signaling pathways may also impact the regulation of AQP6 in the kidney.

How is the expression of AQP6 regulated in the kidney?

The expression of AQP6 in the kidney is regulated by several factors. One important regulator is the hormone vasopressin, also known as antidiuretic hormone (ADH). Vasopressin acts on the renal collecting ducts and upregulates the transcription and expression of AQP2, which is closely related to AQP6. AQP2 and AQP6 share some regulatory mechanisms, and studies have shown that vasopressin can also influence AQP6 expression. Additionally, other factors such as pH levels, electrolyte balance, and hormonal signaling pathways may also impact the regulation of AQP6 in the kidney.

Can AQP6 be targeted for therapeutic interventions?

AQP6's unique properties and role in kidney function make it an intriguing target for therapeutic interventions. However, since AQP6 has complex interactions and its precise roles are still being elucidated, developing specific drugs targeting AQP6 remains a challenge.

Are there any diseases or conditions associated with dysfunction of AQP6?

Although relatively less well-studied than other aquaporins, dysfunction or mutations in AQP6 have been associated with recessive distal renal tubular acidosis (dRTA). In individuals with dRTA, there is impaired acid secretion in the distal tubules of the kidney, leading to an inability to effectively excrete acid and maintain proper acid-base balance. dRTA is characterized by metabolic acidosis and can result in symptoms such as electrolyte imbalances, growth abnormalities, and kidney stone formation.

Are there any diseases or conditions associated with dysfunction of AQP6?

Although relatively less well-studied than other aquaporins, dysfunction or mutations in AQP6 have been associated with recessive distal renal tubular acidosis (dRTA). In individuals with dRTA, there is impaired acid secretion in the distal tubules of the kidney, leading to an inability to effectively excrete acid and maintain proper acid-base balance. dRTA is characterized by metabolic acidosis and can result in symptoms such as electrolyte imbalances, growth abnormalities, and kidney stone formation.

How does AQP6 contribute to water and solute transport in the kidney?

AQP6 is predominantly expressed in the intercalated cells of the renal collecting ducts in the kidney. It plays a role in regulating water and solute transport in this part of the nephron. AQP6 acts as a water channel and allows for the movement of water across the cell membrane, facilitating the reabsorption or excretion of water depending on the needs of the body.

How does AQP6 contribute to water and solute transport in the kidney?

AQP6 is predominantly expressed in the intercalated cells of the renal collecting ducts in the kidney. It plays a role in regulating water and solute transport in this part of the nephron. AQP6 acts as a water channel and allows for the movement of water across the cell membrane, facilitating the reabsorption or excretion of water depending on the needs of the body.

Are there any therapeutic approaches targeting AQP6 indirectly, such as through downstream signaling pathways?

While there are currently no direct therapeutic approaches targeting AQP6, some research has focused on modulating downstream signaling pathways that could indirectly affect AQP6 function. For example, targeting signaling molecules like vasopressin or angiotensin II, which regulate AQP6 expression, could potentially alter AQP6 activity in a therapeutic context. However, further research is needed to explore the feasibility and potential effectiveness of these indirect therapeutic approaches.

Are there any therapeutic approaches targeting AQP6 indirectly, such as through downstream signaling pathways?

While there are currently no direct therapeutic approaches targeting AQP6, some research has focused on modulating downstream signaling pathways that could indirectly affect AQP6 function. For example, targeting signaling molecules like vasopressin or angiotensin II, which regulate AQP6 expression, could potentially alter AQP6 activity in a therapeutic context. However, further research is needed to explore the feasibility and potential effectiveness of these indirect therapeutic approaches.

Is there any potential for therapeutic targeting of AQP6 in kidney disorders?

The understanding of AQP6's role in kidney function and its potential involvement in renal disorders is still evolving. While there are currently no approved therapies directly targeting AQP6, advancements in research may uncover potential therapeutic strategies. For example, modulating AQP6 expression or function could potentially be explored as a therapeutic approach for addressing disorders like dRTA. However, more research is needed to fully understand the underlying mechanisms and potential treatment options targeting AQP6 in kidney disorders.

Is there any potential for therapeutic targeting of AQP6 in kidney disorders?

The understanding of AQP6's role in kidney function and its potential involvement in renal disorders is still evolving. While there are currently no approved therapies directly targeting AQP6, advancements in research may uncover potential therapeutic strategies. For example, modulating AQP6 expression or function could potentially be explored as a therapeutic approach for addressing disorders like dRTA. However, more research is needed to fully understand the underlying mechanisms and potential treatment options targeting AQP6 in kidney disorders.

Are there any drugs or therapies that target AQP6?

Currently, there are no specific drugs or therapies targeting AQP6. However, understanding the regulation and function of AQP6 may provide valuable insights for the development of future treatments for water balance disorders or renal diseases.

Are there any known mutations or variations in the AQP6 gene associated with diseases?

To date, no disease-specific mutations in the AQP6 gene have been identified. However, variations in AQP6 expression and function have been implicated in renal disorders such as nephrogenic diabetes insipidus, which affects the ability to concentrate urine.

What are the consequences of AQP6 dysfunction?

Dysfunction or dysregulation of AQP6 can lead to impaired water reabsorption in the kidney, potentially contributing to disorders such as nephrogenic diabetes insipidus. Altered AQP6 expression has also been associated with urolithiasis, or the formation of kidney stones.

How is the structure of AQP6 protein organized?

AQP6 protein consists of six transmembrane helices that span the cell membrane, with N- and C-termini located on the cytoplasmic side. These helices create a water-conducting pore through which water molecules can pass.

How is AQP6 affected by different environmental factors?

AQP6 activity can be influenced by various environmental factors. For example, studies have shown that changes in pH levels can affect AQP6 gating, leading to alterations in water transport. Additionally, certain ions, such as calcium and potassium, have been found to modulate AQP6 function. Exposure to different chemicals or drugs may also affect AQP6 activity, although more research is needed to understand the specific mechanisms of these interactions.

How is AQP6 affected by different environmental factors?

AQP6 activity can be influenced by various environmental factors. For example, studies have shown that changes in pH levels can affect AQP6 gating, leading to alterations in water transport. Additionally, certain ions, such as calcium and potassium, have been found to modulate AQP6 function. Exposure to different chemicals or drugs may also affect AQP6 activity, although more research is needed to understand the specific mechanisms of these interactions.

Can AQP6 have any potential implications in conditions other than kidney disorders?

Although AQP6 is primarily studied in the context of renal function, its expression has also been detected in other tissues, including the brain and reproductive organs. Further research is needed to fully understand the role of AQP6 in these tissues and its potential implications in related conditions or diseases.

Are there any known interactions with other proteins or molecules affecting AQP6 activity?

AQP6 may interact with other proteins or molecules within the cell membrane, influencing its activity. For example, certain phospholipids have been found to modulate AQP6 function. Further research is needed to fully understand these interactions and their impact on AQP6 activity.

Are there any ongoing studies or research focused on AQP6?

Yes, ongoing research is focused on further understanding the role of AQP6 in kidney function, its regulation, and potential implications in related disorders. Additionally, efforts are being made to uncover its presence and functions outside the kidney in different organs and tissues.

Are there any ongoing studies or research focused on AQP6?

Yes, ongoing research is focused on further understanding the role of AQP6 in kidney function, its regulation, and potential implications in related disorders. Additionally, efforts are being made to uncover its presence and functions outside the kidney in different organs and tissues.