Recombinant Full Length Mouse E3 Ubiquitin-Protein Ligase Rnf133(Rnf133) Protein, His-Tagged

• Cat.No.: RFL10179MF
• Product Overview: Recombinant Full Length Mouse E3 ubiquitin-protein ligase RNF133(Rnf133) Protein (Q14B02) (1-382aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Mus musculus (Mouse)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-382)
• AA Sequence: MNPLQTSTWQNQAPSFWLLRFSFIW LVSQKCCTASAVWTAYMNISFHVGN RMLSELGETG VFGRSSILKRVAGVVVPPEGKIQNA CDPNTTFILPRNKEPWIALIERGGC AFTQKIKVAS EHGARGVIIYNFPGTGNQVFPMSHQ AFEDIVVVMIGNIKGMEILHLIRKG VHVTVMVEVG RKHVIWLNHYFVSFMIVTTATLAYF TFYHIRRLWVARIENRRWKRLTREL KKAFGQLQVR VLKEGDEEVNPNADSCVICFEAYKP NEIVRILTCKHFFHKNCIDPWILAH GTCPMCKCDI LKALGIQMDIEDGTDSLQVLMSNEL PGTLSPVEEETNYELPPARTSSKVT HVQEHPTSSA NAGSQPPEAEETSHPSHGQQVL
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: Rnf133
• Synonyms: Rnf133; Greul2; E3 ubiquitin-protein ligase RNF133; Goliath-related E3 ubiquitin-protein ligase 2; RING finger protein 133; RING-type E3 ubiquitin transferase RNF133
• UniProt ID: Q14B02

Gene Information

• Gene Name: Rnf133
• Synonyms: Rnf133; Greul2; E3 ubiquitin-protein ligase RNF133; Goliath-related E3 ubiquitin-protein ligase 2; RING finger protein 133; RING-type E3 ubiquitin transferase RNF133
• UniProt ID: Q14B02

Reviews

02/16/2022

Easy to purify and manipulate, easy to store, and easy to use.

10/05/2020

The results are reliable and trustworthy.

07/25/2020

RNF133 is easy to prepare, so we can easily prepare it ourselves.

Q&As

Does RNF133 interact with other proteins or molecules?

The interaction of RNF133 with other proteins or molecules is not fully understood. However, based on its structural and functional characteristics, it can be speculated that it may interact with some enzymes, transcription factors, or other signal transduction molecules to participate in the intracellular protein degradation process.

Can RNF133 mutations affect cells?

Mutations in RNF133 may have a variety of effects on cells, such as affecting intracellular protein degradation, leading to abnormal cell proliferation, and inducing apoptosis. These effects may be related to the occurrence of some diseases, such as neurological diseases, cancer, etc.

How can the interaction network of RNF133 be studied?

Molecular biology and biochemical methods, such as yeast two-hybridization, affinity chromatography, mass spectrometry, etc., can be used to study the interaction network of RNF133. These methods can help us understand the function and mechanism of action of RNF133 in cells.

Is there an application of RNF133 in disease diagnosis and treatment?

RNF133 can be used as a biomarker for disease diagnosis and as a therapeutic target. For example, by detecting the expression levels or mutations of RNF133, doctors can be able to diagnose and evaluate the prognosis of certain diseases, and treatment strategies for RNF133 can also provide patients with more effective treatment options.

What factors are associated with RNF133 expression levels?

The expression level of RNF133 may be affected by a variety of factors, such as gene mutations, transcription factors, nutrients, etc. These factors may regulate the expression level of RNF133 by influencing its transcription and translation processes.

How to construct cell models with RNF133 overexpression or knockdown?

Cell models with overexpression or knockout of RNF133 can be established by gene transfection. The vector of the gene of interest or a specific knockout gene is introduced into the cells, and the stably expressed or knocked out cell line is obtained through screening and identification.