Description : |
Enables several functions, including E-box binding activity; Krueppel-associated box domain binding activity; and promoter-specific chromatin binding activity. Involved in several processes, including animal organ development; histone deacetylation; and regulation of gene expression. Acts upstream of or within several processes, including negative regulation of signal transduction; negative regulation of transcription by RNA polymerase II; and nervous system development. Located in heterochromatin; neuronal cell body; and nucleus. Part of NuRD complex and transcription repressor complex. Is expressed in several structures, including brain; early conceptus; embryo mesenchyme; genitourinary system; and sensory organ. Orthologous to human HDAC1 (histone deacetylase 1). |
Source : |
Insect cells |
Species : |
Mouse |
Tag : |
C-Flag,C-His |
Protein length : |
1-482 aa |
Form : |
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 100ug/ml flag peptide. |
Bio-activity : |
≥460 pmol/min/μg. |
Molecular Mass : |
57 kDa |
Purity : |
≥50% |
Applications : |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Storage : |
Store at -80 centigrade. |
Storage Buffer : |
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 3, and Mouse HDAC1 (0.3-20 ng). Incubation condition: 30 min at 37°C followed by developing for 15 min at room temperature. |
Use/Stability : |
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles. |
References : |
1. Rikimaru, T. et al., Oncology 72(1-2), 69-74 (2007). 2. Ishihama, K. et al., J Clin Pathol 60(11), 1205-10 (2007). |