Recombinant Full Length Pig Translocator Protein(Tspo) Protein, His&Myc-Tagged

• Cat.No.: RFL35428SF
• Product Overview: Recombinant Full Length Pig Translocator protein(TSPO) Protein (Q6UN27) (1-169aa), fused to N-terminal 10xHis-SUMO tag and C-terminal Myc tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Sus scrofa (Pig)
• Tag: His&Myc
• Form: Lyophilized powder
• Protein Length: Full Length (1-169aa)
• AA Sequence: MAPPWLPAVGFTLVPSLGGFLSSRN VLGKGLHWYAGLQKPSWHPPHWTLA PIWGTLYSAMGYGSYMIWKELGGFS EEAVVPLGLYAGQLALNWAWPPLFF GARQMGWALVDLVLTGGVAAATAVA WYQVSPLAARLLYPYLAWLAFAATL NYCVWRDNQGRRGGRRPSE Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: TSPO
• Synonyms: TSPO; BZRP; Translocator protein; Peripheral-type benzodiazepine receptor; PBR
• UniProt ID: Q6UN27

Gene Information

• Gene Name: TSPO
• Synonyms: TSPO; BZRP; Translocator protein; Peripheral-type benzodiazepine receptor; PBR
• UniProt ID: Q6UN27

Reviews

01/28/2023

Prolonged transportation will not affect the stability of the product.

07/11/2021

The price of this one is very reasonable, and the quality is very good, I am very satisfied.

02/13/2020

It has high solubility and can be dissolved quickly.

Q&As

How does TSPO affect neuroprotection?

TSPO has an impact on neuroprotection by modulating mitochondrial function to affect the survival and death of nerve cells. Specific mechanisms include inhibition of apoptosis and alleviation of oxidative stress.

What are the challenges and prospects of current research on TSPO?

At present, the research on TSPO faces some challenges, such as the difference in the expression of TSPO in various tissues, the functional changes of TSPO in different disease states, and the interaction between TSPO and other molecules. However, with the continuous advancement of technology and the in-depth understanding of the functions of TSPO, it is expected that there will be greater breakthroughs in drug design and treatment strategies for TSPO in the future.

How is TSPO involved in the synthesis and transport of cholesterol?

This protein regulates the permeability of mitochondrial membranes to cholesterol by binding to cholesterol transporters, thereby affecting cholesterol synthesis and trafficking.

Is TSPO associated with a variety of diseases,

Since TSPO is associated with a variety of diseases,it may be a therapeutic target for a variety of diseases, such as neurodegenerative diseases, cancer, and mental disorders. Some progress has been made in the design and development of drugs for TSPO.

How to detect the expression and function of TSPO?

Commonly used TSPO detection methods include immunohistochemistry, western blotting, flow cytometry, etc. In addition, TSPO ligands can also be used for binding experiments or functional experiments to study their biological functions.

What circumstances does the expression level of TSPO change?

The expression level of TSPO changes in a variety of physiological and pathological conditions, such as hypoxia, ischemia, inflammation, aging, etc. These changes affect the function of the mitochondria, which affects the survival and death of cells.