Recombinant Full Length Rat Abhydrolase Domain-Containing Protein 16A(Abhd16A) Protein, His-Tagged

• Cat.No.: RFL32842RF
• Product Overview: Recombinant Full Length Rat Abhydrolase domain-containing protein 16A(Abhd16a) Protein (Q6MG55) (2-558aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Rattus norvegicus (Rat)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length of Mature Protein (2-558)
• AA Sequence: AKLLSCVLGPRLYKIYRERDTDRAA TSVPETPTAVPAASSSSWDSYYQPR ALEKHADSIL ALASVFWSISYYSSPFAFFYLYRKG YLSLSKVVPFSHYAGTLLVLLAGVA CLRGIGRWTN PQYRQFITILEATHRNQSAENKRQL ANYNFDFRSWPVDFHWEEPSSRKGS RGGPSRRGVA LLRPEPLHRGTADTFLNRVKKLPCQ ITSYLVAHTLGRRMLYPGSVYLLQK ALMPVLLQGQ ARLVEECNGRRAKLLACDGNEIDTM FVDRRGTAEPQGQKLVICCEGNAGF YEVGCVSTPL EAGYSVLGWNHPGFAGSTGVPFPQN EANAMDVVVQFAIHRLGFQPQDIVI YAWSIGGFTA TWAAMSYPDISAVILDASFDDLVPL ALKVMPDSWRALVTRTVRQHLNLNN AEQLCRFQGP VLLVRRTKDEIITTTVPEDIMSNRG NDLLLKLLQFRYPRVMTEEGLRAVR QWLEASSQLE EASIYSRWEVDEDWCVSVLRSYQAE HGPDFPWSVGEDMSVDGRRQLALFL ARKHLHNFEA THCTPLPAQHFQMPWCL
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: Abhd16a
• Synonyms: Abhd16a; Bat5; Phosphatidylserine lipase ABHD16A; Alpha/beta hydrolase domain-containing protein 16A; Abhydrolase domain-containing protein 16A; HLA-B-associated transcript 5 homolog; Monoacylglycerol lipase ABHD16A
• UniProt ID: Q6MG55

Gene Information

• Gene Name: Abhd16a
• Synonyms: Abhd16a; Bat5; Phosphatidylserine lipase ABHD16A; Alpha/beta hydrolase domain-containing protein 16A; Abhydrolase domain-containing protein 16A; HLA-B-associated transcript 5 homolog; Monoacylglycerol lipase ABHD16A
• UniProt ID: Q6MG55

Reviews

04/19/2019

Always good

11/12/2018

5 stars for serivce for sure!

08/13/2018

Suitable price, great!

Q&As

How to prove the ABHD16A is enriched at the ER membrane?

Using STED microscopy, we can found that mammalian ABHD12 is enriched at the ER membrane30, then checked for the co-localization of ABHD16A with ABHD12. Indeed, we found in Neuro-2a cells, that ABHD16A co-localizes with ABHD12, further confirming that ABHD16A is enriched at the ER membrane.

What disease caused by the ABHD16A when it loss the function?

A complicated hereditary spastic paraplegia (HSP) caused by ABHD16A loss of function pathogenic variants and the subsequent altered phosphatidylserine (PS) metabolism.

What lab results suggested that ABHD16A is an integral membrane lipase with a cytosolically oriented active site?

The protein deglycosylation studies and the detergent-independent sensitivity of ABHD16A to proteolytic degradation, have suggested this point.

Could please briefly introduce the enzyme regulation of the ABHD16A?

Inhibited by beta-lactone-based lipid inhibitors, such as beta-lactone palmostatin-B

What are the top 5 interacting proteins for the ABHD16A gene?

They are DTX2, RNF5, AGPAT3, TMEM147, and FAM134C.

What is the biological function of the ABHD16A protein when it exist in the human brain?

ABHD16A is a 21-exon gene which encodes for the α/β hydrolase domain-containing 16A (ABHD16A) enzyme, which is the main brain phosphatidylserine (PS) hydrolase.

What are two main enzymes responsible for the brain Lipopolysaccharides (LPS) metabolism?

ABHD16A and ABHD12, are the main enzymes responsible for the brain LPS metabolism, as ABHD16A generates LPS while ABHD12 catabolizes them into fatty acid and phosphoserine.

What are the effects of ABHD16A protein on humans based on those urrent research results?

ABHD16A PS lipase deficiency results in intellectual disability, progressive spasticity, abnormal corpus callosum, and white matter anomalies, most likely through the dysregulation of lipid species including lyso-PS.

What is the difference between ABHD12 and ABHD16A in mouse brain?

We found relative to the tubulin protein loading control, that ABHD16A was maximally enriched in the cerebellum, and the olfactory bulb, while ABHD12 is ubiquitously expressed at near equal levels in all the aforementioned brain regions.

How does the ABHD16A express in the murine brain?

ABHD16A has a heterogeneous expression in the murine brain and is enriched in the cerebellum, particularly in the granular layer and the olfactory bulb.