Source : |
E. coli |
Species : |
HIV |
Tag : |
His |
Predicted N Terminal : |
Met |
Form : |
Lyophilized from sterile 50 mM Tris, 250 mM Nacl, 1 mM EDTA, 50 % glycerol, pH 7.0. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Molecular Mass : |
The recombinant p51 (Reverse Transcriptase) of human immunodeficiency virus gag-pol consists 458 amino acids and predicts a molecular mass of 53.6 kDa. |
Protein length : |
Pro588-Phe1027 |
Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
Purity : |
> 90 % as determined by SDS-PAGE. |
Storage : |
Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Official Symbol : |
gag-pol |
Synonyms : |
Gag-Pol;Gag-Pol polyprotein;aspartic peptidase;p66 subunit;integrase;Gag-Pol Transframe peptide;Pol;reverse transcriptase p51 subunit |