Recombinant Homo sapiens ADAT1

• Cat.No.: ADAT1-3157H
• Product Overview: Adenosine Deaminase, tRNA-specific 1

Specification

• Description: This gene is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, proteins encoded by these genes participate in the pre-mRNA editing of nuclear transcripts. The protein encoded by this gene, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA. Alternatively spliced transcript variants have been described.
• Source: E. Coli
• Species: Homo sapiens
• Form: Supplied as a liquid in PBS, pH 7.2. No preservative added.
• Purity: Highly PurifiedHighly purified
• Applications: Suitable for use in ELISA as an Antigen, Ligand, or Assay Standard.
• Notes: Small volumes of ADAT1 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: -70 degree C
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: ADAT1 adenosine deaminase, tRNA-specific 1 [ Homo sapiens ]
• Official Symbol: ADAT1
• Synonyms: ADAT1; HADAT1; tRNA-specific adenosine deaminase 1; adenosine deaminase acting on Trna; tRNA-specific adenosine-37 deaminase
• Gene ID: 23536
• mRNA Refseq: NM_012091
• Protein Refseq: NP_036223
• MIM: 604230
• UniProt ID: Q9BUB4
• Chromosome Location: 16q23.1
• Function: RNA binding; metal ion binding; tRNA-specific adenosine deaminase activity

Q&As

How is ADAT1 gene expression regulated?

ADAT1 gene expression is mainly regulated at the transcriptional level. Some studies suggest that the ADAT1 promoter region is regulated by several transcription factors, including Sp1, EGR1 and WT1. Additionally, some studies indicate that ADAT1 expression can be regulated by post-transcriptional mechanisms such as miRNA regulation. The stability of ADAT1 protein and mRNA transcripts is also influenced by a variety of protein and RNA binding factors, indicating the complex nature of ADAT1 gene expression regulation.

Can ADAT1 protein have agricultural applications?

Yes, ADAT1 protein could have agricultural applications as it influences the plant growth process. The ADAT1 gene plays a role in stabilizing specific tRNAs in plants, thereby influencing the cellular response to stress and nutrient availability. Additionally, in some species, ADAT1 is known to be regulated by environmental factors, such as light and temperature. Therefore, ADAT1 protein could potentially be utilized as a tool to enhance crop growth and better adapt plants to specific environmental conditions.

Is ADAT1 protein being used in biotechnology research?

ADAT1 protein is being used in biotechnology research, particularly in the study of RNA editing processes. Its role in stabilizing specific tRNAs is also being studied for its potential applications in enhancing crop growth and improving stress tolerance. Additionally, ADAT1 expression is being investigated as a potential biomarker in various diseases, including cancer.

Is there any connection between ADAT1 protein and cancer?

Yes, ADAT1 has been associated with cancer as it plays a critical role in RNA editing processes that influence the prognosis and severity of various malignancies. For instance, it has been observed that ADAT1 expression is elevated in some tumor cells, and its overexpression can be associated with a poor prognosis. However, the exact mechanism of its role in cancer is not yet fully understood.

How can ADAT1 protein be used to enhance crop growth?

ADAT1 protein could potentially be utilized as a tool to enhance crop growth by regulating the stability of specific tRNAs in plants. By stabilizing tRNAs, ADAT1 could improve the cellular response to stress and nutrient availability, thereby enhancing plant growth. Additionally, as ADAT1 is regulated by environmental factors such as light and temperature, it could be used to help plants better adapt to specific environmental conditions, further improving crop growth.

Can ADAT1 protein be used to develop stress-tolerant crops?

Yes, ADAT1 protein could potentially be used to develop stress-tolerant crops. Its ability to stabilize specific tRNAs in plants could help improve the cellular response to stress conditions, allowing plants to better tolerate drought, high temperatures, and other environmental stresses. Additionally, as ADAT1 expression is regulated by stress processes such as oxidative stress and viral infection, it could be used as a tool to develop crops that are more resilient to stress.

Is ADAT1 protein linked to neurological disorders?

Yes, ADAT1 mutations have been documented as one of the causes of neurological disorders. Some of the syndromes linked to ADAT1 include Aicardi-Goutieres syndrome, Charcot-Marie-Tooth disease (CMT), and Hereditary Spastic Paraplegia (HSP). ADAT1 gene has been linked with the survival and maintenance of motor neurons and the alteration in its activity level could result in pathophysiology of these neurons leading to neuropathies.

Can ADAT1 protein be used to develop new drugs?

ADAT1 protein could potentially be used to develop new drugs as its activity is implicated in various cellular processes, including stress response and RNA regulation. However, more research needs to be done to fully understand the protein's functions and how it could be targeted for drug development.

Is ADAT1 protein conserved across different species?

Yes, ADAT1 protein is highly conserved across many different species, including bacteria, yeast, plants, and animals. The ADAT1 protein function is well-conserved, implying its crucial role in evolutionary processes. The high level of conservation of the gene sequence and protein activity indicates that ADAT1 has functional significance in biological processes that are essential for survival across different species.

Are there any inhibitors or activators known for the ADAT1 protein?

Currently, there are no known direct inhibitors or activators of the ADAT1 protein. However, its activity can be indirectly regulated through other cellular mechanisms, such as regulation of tRNA levels or transcriptional regulation of ADAT1 gene itself. Additionally, cellular stress processes such as heat shock, oxidative stress, and viral infection have been known to influence ADAT1 expression, indicating the potential for developing modulators of ADAT1 activity as therapeutics.

How can ADAT1 protein be used in medicine?

ADAT1 protein has potential therapeutic applications due to its essential role in RNA editing mechanisms. RNA editing prevents diseases that could occur due to the misfolding of proteins or the dysregulation of gene expression. Some studies suggest that the restoration of ADAT1 expression can rescue defective RNA editing in certain diseases. Therefore, ADAT1 can be a potential target for RNA editing-based therapies.

Is ADAT1 protein being researched for use in gene therapy?

ADAT1 protein is not currently being researched for use in gene therapy. Its role in stabilizing tRNAs could potentially be used to improve the production of therapeutic proteins in cells, but there are currently no known applications of ADAT1 protein in gene therapy.

Can ADAT1 protein be used as a diagnostic biomarker?

In certain diseases, ADAT1 expression and activity levels are known to be disrupted, indicating its potential for use as a diagnostic biomarker. For example, decreased levels of ADAT1 activity have been linked to malignancies, while mutations in ADAT1 gene have been linked to numerous genetic disorders including hereditary spastic paraplegias (HSP), Aicardi-Goutieres syndrome (AGS), and dyschromatosis symmetrica hereditaria (DSH). However, more research is required to establish its usefulness as a diagnostic biomarker.