Recombinant Human ABCA7 protein, His & GST-tagged

• Cat.No.: ABCA7-1677H
• Product Overview: Recombinant Human ABCA7 aa. (Glu53~Leu249) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This full transporter has been detected predominantly in myelo-lymphatic tissues with the highest expression in peripheral leukocytes, thymus, spleen, and bone marrow. The function of this protein is not yet known; however, the expression pattern suggests a role in lipid homeostasis in cells of the immune system.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: 51.0kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Glu53~Leu249
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 5.2

Gene Information

• Gene Name: ABCA7 ATP binding cassette subfamily A member 7 [ Homo sapiens (human) ]
• Official Symbol: ABCA7
• Synonyms: ABCA7; ATP binding cassette subfamily A member 7; AD9; ABCX; ABCA-SSN; ATP-binding cassette sub-family A member 7; ATP-binding cassette, sub-family A (ABC1), member 7; autoantigen SS-N; macrophage ABC transporter
• Gene ID: 10347
• mRNA Refseq: NM_019112.3
• Protein Refseq: NP_061985.2
• UniProt ID: Q8IZY2

Reviews

06/19/2021

Rich catalog of recombinant protein products, no need to look for protein products everywhere.

07/12/2020

Customizable, very convenient

Q&As

Are your recombinant protein products full-length proteins or protein domains?

Our products have full-length proteins and protein parts, you can check the details in the product overview.

What biological processes are ABCA7 involved in?

ABCA7 is involved in lipid transport, phagocytosis, visual learning, and positive regulation of cholesterol efflux.

Do you have biotinylated ABCA7 protein products?

We do. Our products can be customized according to different needs of customers, and the labels that can be added include biotin, fluorescein, alexa flour, APC, etc.

How does abca7 contribute to AD-related pathology?

ABCA7 loss of function significantly increases amyloid precursor protein proteolysis and amyloid-β production.

Which expression systems can be used to express the abca7 gene?

Expression systems such as HEK293 and CHO can be used.

Can a GFP tag be added to study protein function?

Some studies added green fluorescent protein to the C-terminus of ABCA7, which did not affect the lipid release reaction.

Is the ABCA7 variant a susceptibility locus for late-onset AD?

Cumulative genetic evidence collected through genome-wide studies (GWAS) has identified ABCA7 variants as late-onset AD susceptibility loci, including rs3764650, rs3752246, and rs115550680.

How ABCA7 limits amyloid-beta production?

ABCA7 limits amyloid-β production by regulating amyloid-β A4 precursor protein endocytosis and/or processing.

Does ABCA7 transport phosphatidylcholine?

it does. But when phosphatidylcholine and phosphatidylserine are present at the same time, ABCA7 will preferentially transport phosphatidylserine.