Recombinant Human ABCC2

• Cat.No.: ABCC2-2537H
• Product Overview: Recombinant Human ABCC2 was produced inSf9 cells.

Specification

• Cat. No.: ABCC2-2537H
• Description: MRP2 (ABCC2) is an organic anion transporter found in the liver, kidney, and gut epithelium apical membranes. The transport of glucuronate conjugates plays a role in the detoxification of endogenous and xenobiotic substances, and may cause multidrug resistance (MDR) in tumor cells.
• Source: Sf9 cells.
• Form: Supplied as isolated Sf9 cell membranes containing human MRP2 suspended in TMEP solution.
• Biochem/physiol Actions: The vesicular transport assay determines the interaction of compounds with the MRP2 transporter. The interaction is detected by changes in the initial rate of 3H-β-estradiol 17-(β-D-glucuronide) transport by MRP2 into membrane vesicles purified from Sf9 cells expressing the transporters. Membrane preparations from infected cells always contain some closed membrane vesicles that have an inside-out orientation (5-10% of total lipid). In the case of these inside-out vesicles, transport of substrates across the membrane takes molecules from the surrounding buffer and transports them into the vesicles.
• Analysis Note: The quantity of transported molecules can be determined by methods such as HPLC, LC/MS/MS separation and detection, and also by labeling with fluorescent or radioactive (3H-β-estradiol 17-(β-D-glucuronide) tags. MRP2 mediates the transport of β-estradiol 17-(β-D-glucuronide) (E217βG) very efficiently. Compounds that interact with the transporter modulate the initial rate of E217βG transport measured without any other compounds added. If a substance is a transported substrate of the transporter, it might compete with E217βG, thus reducing the rate of E217βG transport. If a compound is an inhibitor of the transporter, it will block the transport of E217βG into the membrane vesicles. Some compounds can be co-transported with E217βG increasing the rate of E217βG transport compared to the control level.
• Storage: −70°C.

Gene Information

• Gene Name: ABCC2 ATP-binding cassette, sub-family C (CFTR/MRP), member 2 [ Homo sapiens ]
• Synonyms: ABCC2; ATP-binding cassette, sub-family C (CFTR/MRP), member 2;ABC30; CMOAT; DJS; KIAA1010; MRP2; cMRP; OTTHUMP00000020267;canalicular multispecific organic anion transporter; Canalicular multispecific organic anion transporter 1;Multidrug resistance-associated protein 2; Canalicular multidrug resistance protein; CMOAT1; ATP-binding cassette sub-family C member 2
• Gene ID: 1244
• mRNA Refseq: NM_000392
• Protein Refseq: NP_000383
• MIM: 601107
• UniProt ID: Q92887
• Chromosome Location: 10q24
• Pathway: ABC transporters - General
• Function: ATP binding; ATPase activity; coupled to transmembrane movement of substances;nucleotide binding; organic anion transmembrane transporter activity; protein binding; transporter activity

Reviews

02/01/2023

Complete packaging and fast transportation

01/09/2022

Good positioning effect

04/23/2021

Less clutter and clear background

Q&As

Can Cry1Ca reduce the drug resistance of ABCC2？

Cry1Ca is a protein that belongs to the family of crystal (Cry) proteins produced by the bacterium Bacillus thuringiensis.Some reseachs show that Cry1Ca could decrease the transport function of ABCC2 in plant.

Can I use antibody for abcc2 to distinguish hepatocytes from other cells？

What is the relationship between the genetic variation of transporter ABCC2 and drug-induced hyperbilirubinemia

Carriage of the ABCC2 (MRP2) rs717620 T variant may increase the risk of hyperbilirubinemia and mortality in patients with DILI.

Is Dubin Johnson's syndrome hereditary?

Yes, Dubin-Johnson syndrome (DJS) is a hereditary condition, which means it is passed down through families.

What are the causes for dubin johnson syndrome?

Causes Dubin-Johnson syndrome is caused by changes in a gene known as ABCC2. The ABCC2 gene provides instructions for making a protein that transports certain substances out of cells so they can be released (excreted) from the body.

What transport does ABCC2 generally mediate

ABCC2 and ABCC3 (also known as MRP2 and MRP3) mediate the transport of various conjugated organic anions, including many drugs, toxicants and endogenous compounds.

Where does ABCC2 most expression?

ABCC2/Abcc2 (MRP2/Mrp2) is expressed at major physiological barriers, such as the canalicular membrane of liver cells, kidney proximal tubule epithelial cells, enterocytes of the small and large intestine, and syncytiotrophoblast of the placenta.

What molecules can ABCC2 generally transport?

ABCC2/Abcc2 transports a variety of amphiphilic anions that belong to different classes of molecules, such as endogenous compounds (e.g., bilirubin-glucuronides), drugs, toxic chemicals, nutraceuticals, and their conjugates.

What's the polymorphism risk of ABCC2 c.-24C>T

The ABCC2 c.-24C>T polymorphism is a likely risk factor for resistance to AEDs.

Does ABCC2 polymorphism reduce antiepileptic drug resistance?

Some researchs show that the ABCC2 G1249A polymorphism is significantly associated with a decreased risk of AED resistance. However, further functional investigations are warranted to validate the association.

What diseases will ABCC2 defects cause

Acquired or hereditary deficiency of ABCC2, the latter known as Dubin-Johnson syndrome in humans

What are the effects of an ABCC2 abnormality?

It is possible that ABCC2/Abcc2 transport activity as well as inhibitor sensitivity could lead to a number of phenomena (e.g. a difference in the biliary excretion clearance, a delay in the elimination half-life from the circulating blood and toxic side effects on ABCC2 -mediated drug-drug interactions, such as drug-induced hyperbilirubinemia).

What drug resistance does ABCC2 cause

Human ABCC2 is an ATP-binding cassette transporter involved in the export of endobiotics and xenobiotics. It is involved in cisplatin resistance in cancer cells, particularly in ovarian cancer.

In which cells ABCC2 generally exists

ABCC2 is a member of the multidrug resistance protein subfamily localized exclusively to the apical membrane domain of polarized cells, such as hepatocytes, renal proximal tubule epithelia, and intestinal epithelia.

What's the role of ABCC2 in liver

The hepatic ABCC2 pump contributes to the driving forces of bile flow.