Recombinant Human ABCC2 protein, His-tagged

• Cat.No.: ABCC2-8117H
• Product Overview: Recombinant Human ABCC2 aa. (Ile1300~Glu1534) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ile1300~Glu1534
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.1.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: ABCC2 ATP binding cassette subfamily C member 2 [ Homo sapiens (human) ]
• Official Symbol: ABCC2
• Synonyms: ABCC2; ATP binding cassette subfamily C member 2; DJS; MRP2; cMRP; ABC30; CMOAT
• Gene ID: 1244
• mRNA Refseq: NM_000392.4
• Protein Refseq: NP_000383.1
• UniProt ID: Q92887

Reviews

02/01/2023

Complete packaging and fast transportation

01/09/2022

Good positioning effect

04/23/2021

Less clutter and clear background

Q&As

Can Cry1Ca reduce the drug resistance of ABCC2？

Cry1Ca is a protein that belongs to the family of crystal (Cry) proteins produced by the bacterium Bacillus thuringiensis.Some reseachs show that Cry1Ca could decrease the transport function of ABCC2 in plant.

Can I use antibody for abcc2 to distinguish hepatocytes from other cells？

What is the relationship between the genetic variation of transporter ABCC2 and drug-induced hyperbilirubinemia

Carriage of the ABCC2 (MRP2) rs717620 T variant may increase the risk of hyperbilirubinemia and mortality in patients with DILI.

Is Dubin Johnson's syndrome hereditary?

Yes, Dubin-Johnson syndrome (DJS) is a hereditary condition, which means it is passed down through families.

What are the causes for dubin johnson syndrome?

Causes Dubin-Johnson syndrome is caused by changes in a gene known as ABCC2. The ABCC2 gene provides instructions for making a protein that transports certain substances out of cells so they can be released (excreted) from the body.

What transport does ABCC2 generally mediate

ABCC2 and ABCC3 (also known as MRP2 and MRP3) mediate the transport of various conjugated organic anions, including many drugs, toxicants and endogenous compounds.

Where does ABCC2 most expression?

ABCC2/Abcc2 (MRP2/Mrp2) is expressed at major physiological barriers, such as the canalicular membrane of liver cells, kidney proximal tubule epithelial cells, enterocytes of the small and large intestine, and syncytiotrophoblast of the placenta.

What molecules can ABCC2 generally transport?

ABCC2/Abcc2 transports a variety of amphiphilic anions that belong to different classes of molecules, such as endogenous compounds (e.g., bilirubin-glucuronides), drugs, toxic chemicals, nutraceuticals, and their conjugates.

What's the polymorphism risk of ABCC2 c.-24C>T

The ABCC2 c.-24C>T polymorphism is a likely risk factor for resistance to AEDs.

Does ABCC2 polymorphism reduce antiepileptic drug resistance?

Some researchs show that the ABCC2 G1249A polymorphism is significantly associated with a decreased risk of AED resistance. However, further functional investigations are warranted to validate the association.

What diseases will ABCC2 defects cause

Acquired or hereditary deficiency of ABCC2, the latter known as Dubin-Johnson syndrome in humans

What are the effects of an ABCC2 abnormality?

It is possible that ABCC2/Abcc2 transport activity as well as inhibitor sensitivity could lead to a number of phenomena (e.g. a difference in the biliary excretion clearance, a delay in the elimination half-life from the circulating blood and toxic side effects on ABCC2 -mediated drug-drug interactions, such as drug-induced hyperbilirubinemia).

What drug resistance does ABCC2 cause

Human ABCC2 is an ATP-binding cassette transporter involved in the export of endobiotics and xenobiotics. It is involved in cisplatin resistance in cancer cells, particularly in ovarian cancer.

In which cells ABCC2 generally exists

ABCC2 is a member of the multidrug resistance protein subfamily localized exclusively to the apical membrane domain of polarized cells, such as hepatocytes, renal proximal tubule epithelia, and intestinal epithelia.

What's the role of ABCC2 in liver

The hepatic ABCC2 pump contributes to the driving forces of bile flow.