Recombinant Human ABCF2 protein, His & T7-tagged

• Cat.No.: ABCF2-8137H
• Product Overview: Recombinant Human ABCF2 aa. (Ile396~Val623 (Accession # Q9UG63)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.9kDa.
• Protein length: Ile396~Val623 (Accession # Q9UG63)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.2.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: ABCF2 ATP binding cassette subfamily F member 2 [ Homo sapiens (human) ]
• Official Symbol: ABCF2
• Synonyms: ABCF2; ATP binding cassette subfamily F member 2; ABC28; HUSSY18; HUSSY-18; EST133090; ATP-binding cassette sub-family F member 2; ABC-type transport protein; ATP-binding cassette, sub-family F (GCN20), member 2; iron-inhibited ABC transporter 2
• Gene ID: 10061
• mRNA Refseq: NM_005692.4
• Protein Refseq: NP_005683.2
• UniProt ID: Q9UG63

Reviews

08/30/2021

The protein product readily dissolved in the recommended buffer, saving me time and effort during preparation

06/27/2019

The functional assay involving the protein product revealed a dose-dependent response, confirming its potency and reliability in our study

04/06/2018

Considering the superior quality and performance, the protein product's price was reasonable.

02/16/2018

I was impressed by the stability of the protein throughout the shipping and storage process

11/03/2017

It ensured that I had a positive experience.

Q&As

Is ABCF2 involved in any specialized cellular processes or pathways?

ABCF2 is involved in specialized cellular processes, particularly in the transport and elimination of substrates from cells. Its primary role is to confer resistance against a wide range of compounds by facilitating their efflux from cells. Additionally, ABCF2 has been implicated in cellular detoxification processes and protection against oxidative stress.

Does ABCF2 play a role in drug resistance or multidrug resistance in cancer cells?

ABCF2 has been implicated in drug resistance or multidrug resistance in cancer cells. It is believed that ABCF2 plays a role in the efflux of chemotherapeutic drugs from cancer cells, reducing their effectiveness. By expelling drugs from cells, ABCF2 can contribute to resistance against multiple drugs and hinder successful cancer treatment.

Are there any known genetic polymorphisms or mutations in the ABCF2 gene that have been associated with altered transporter function or disease susceptibility?

it is possible that variations in the ABCF2 gene could impact its expression levels or regulatory elements, potentially influencing transporter function. 

Are there any specific tissues or organs where ABCF2 is highly expressed or plays a critical role?

It is particularly abundant in tissues with high metabolic activity, such as the liver and kidney, where it is involved in the elimination of potentially toxic compounds.

What is the cellular function of ABCF2?

The cellular function of ABCF2, also known as MRP2 (Multidrug Resistance-Associated Protein 2), is primarily related to the transport of molecules across cellular membranes

Are there any known physiological or pathological conditions that result in dysregulation of ABCF2 expression or function?

It is plausible that conditions involving altered drug sensitivity, impaired detoxification, or disrupted cellular homeostasis could potentially be associated with dysregulation of ABCF2

How is the expression of ABCF2 regulated at the transcriptional level?

Transcription factors and signaling pathways play a role in regulating its expression. 

Are there any known physiological or pathological conditions that result in altered ABCF2 expression or activity?

it is plausible that conditions involving altered drug sensitivity, impaired cellular detoxification, or disrupted transport processes could potentially be associated with ABCF2 dysregulation

Which species are the portein of ABCD4 present in?

The popular organisms of ABCF2 are human, rat, A.thaliana, mouse and zebrafish.

Does ABCF2 play a role in intracellular transport or vesicle trafficking?

ABCF2 is primarily associated with intracellular transport processes rather than intracellular vesicle trafficking. It functions as an ATP-dependent efflux pump, transporting substrates across cellular membranes, including the plasma membrane and intracellular organelles.

which cellular processes does ABCF2 participate in?

ABCF2 belongs to the ATP-binding cassette (ABC) transporter superfamily and is involved in the efflux of a variety of substrates, including drugs, toxins, and endogenous compounds, from cells.

What is the role of ABCF2 in cellular detoxification pathways, and how does it contribute to the elimination of toxic compounds?

ABCF2 plays a crucial role in cellular detoxification pathways by facilitating the efflux of potentially toxic compounds from cells. It contributes to the elimination of substances such as drugs, toxins, and endogenous metabolites, thereby maintaining cellular homeostasis and protecting cells from potential damage.

Does ABCF2 have any regulatory roles in cellular processes beyond its function as a transporter?

Apart from its function as a transporter, ABCF2 may have regulatory roles in cellular processes. For example, it may modulate the cellular response to oxidative stress or play a role in cellular detoxification pathways.

Are there any known physiological or pathological conditions that result in altered ABCF2 expression or activity?

ABCF2 is believed to be expressed in various tissues and cell types, suggesting a broad expression profile

Are there any genetic variations or mutations in the ABCF2 gene that have been associated with specific diseases or conditions?

Variations or dysregulation of ABCF2 expression could potentially influence drug response or alter cellular homeostasis

Are there any known interacting partners or binding partners of ABCF2?

ABCF2 is known to interact with other transporters, such as ABCC2 (Multidrug Resistance-Associated Protein 2), and with scaffolding proteins involved in regulating its localization and function.

Are there any known substrates or specific classes of molecules that are transported by ABCF2?

ABCF2 is known to transport a wide range of substrates

Are there any post-translational modifications or regulatory mechanisms that modulate ABCF2 activity or function?

Phosphorylation, acetylation, or other modifications could potentially influence its transport activity or interactions with binding partners

Has ABCF2 been implicated in any specific physiological processes, such as metabolism or cellular homeostasis?

It may play a role in cellular metabolism, including the transport of metabolic intermediates or waste products. Additionally, ABCF2 is involved in maintaining cellular homeostasis by eliminating potentially harmful substances from cells.

What is the subcellular localization of ABCF2 and are there any mechanisms that regulate its localization?

ABCF2 is localized to the plasma membrane and is also found in intracellular organelles involved in transport, such as the endoplasmic。 reticulum and mitochondria.