Recombinant Human ABHD1, His-tagged

• Cat.No.: ABHD16A-10144H
• Product Overview: Recombinant Human ABHD1His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Description: A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. The protein encoded by this gene is thought to be involved in some aspects of immunity. Alternatively spliced transcript variants have been described.
• Source: E.coli
• Species: Human
• Tag: His
• Protein length: 209-558a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: ABHD16A abhydrolase domain containing 16A [ Homo sapiens ]
• Official Symbol: ABHD16A
• Synonyms: abhydrolase domain containing 16A; PP199; BAT5; abhydrolase domain-containing protein 16A; NG26; EC 3.-.-.-; D6S82E; G5; HLA-B associated transcript 5; HLA-B-associated transcript 5; Protein G5
• Gene ID: 7920
• mRNA Refseq: NM_021160.2
• Protein Refseq: NP_066983.1
• MIM: 142620
• UniProt ID: O95870
• Chromosome Location: 6p21.3
• Function: hydrolase activity

Reviews

04/19/2019

Always good

11/12/2018

5 stars for serivce for sure!

08/13/2018

Suitable price, great!

Q&As

How to prove the ABHD16A is enriched at the ER membrane?

Using STED microscopy, we can found that mammalian ABHD12 is enriched at the ER membrane30, then checked for the co-localization of ABHD16A with ABHD12. Indeed, we found in Neuro-2a cells, that ABHD16A co-localizes with ABHD12, further confirming that ABHD16A is enriched at the ER membrane.

What disease caused by the ABHD16A when it loss the function?

A complicated hereditary spastic paraplegia (HSP) caused by ABHD16A loss of function pathogenic variants and the subsequent altered phosphatidylserine (PS) metabolism.

What lab results suggested that ABHD16A is an integral membrane lipase with a cytosolically oriented active site?

The protein deglycosylation studies and the detergent-independent sensitivity of ABHD16A to proteolytic degradation, have suggested this point.

Could please briefly introduce the enzyme regulation of the ABHD16A?

Inhibited by beta-lactone-based lipid inhibitors, such as beta-lactone palmostatin-B

What are the top 5 interacting proteins for the ABHD16A gene?

They are DTX2, RNF5, AGPAT3, TMEM147, and FAM134C.

What is the biological function of the ABHD16A protein when it exist in the human brain?

ABHD16A is a 21-exon gene which encodes for the α/β hydrolase domain-containing 16A (ABHD16A) enzyme, which is the main brain phosphatidylserine (PS) hydrolase.

What are two main enzymes responsible for the brain Lipopolysaccharides (LPS) metabolism?

ABHD16A and ABHD12, are the main enzymes responsible for the brain LPS metabolism, as ABHD16A generates LPS while ABHD12 catabolizes them into fatty acid and phosphoserine.

What are the effects of ABHD16A protein on humans based on those urrent research results?

ABHD16A PS lipase deficiency results in intellectual disability, progressive spasticity, abnormal corpus callosum, and white matter anomalies, most likely through the dysregulation of lipid species including lyso-PS.

What is the difference between ABHD12 and ABHD16A in mouse brain?

We found relative to the tubulin protein loading control, that ABHD16A was maximally enriched in the cerebellum, and the olfactory bulb, while ABHD12 is ubiquitously expressed at near equal levels in all the aforementioned brain regions.

How does the ABHD16A express in the murine brain?

ABHD16A has a heterogeneous expression in the murine brain and is enriched in the cerebellum, particularly in the granular layer and the olfactory bulb.