Recombinant Human ABHD10 protein, His-tagged

• Cat.No.: ABHD10-1272H
• Product Overview: Recombinant Human ABHD10 protein(Q9NUJ1)(Thr53-Asn306), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human ABHD10 consists of 271 amino acids and has a calculated molecular mass of 30.3 kDa. The recombinant protein migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
• Protein Length: Thr53-Asn306
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ABHD10 abhydrolase domain containing 10 [ Homo sapiens ]
• Official Symbol: ABHD10
• Synonyms: ABHD10; abhydrolase domain containing 10; abhydrolase domain-containing protein 10, mitochondrial; FLJ11342
• Gene ID: 55347
• mRNA Refseq: NM_018394
• Protein Refseq: NP_060864
• UniProt ID: Q9NUJ1

Reviews

08/28/2019

Suitable price, great!

03/27/2018

Fast shipping and good product.

12/10/2017

Nice performance in electrophoresis.

07/12/2017

Good work in purification process!

Q&As

What kind of chemical compounds can interact with the ABHD10 protein?

 1,3-dinitrobenzene; 2,3,7,8-tetrachlorodibenzodioxine; all-trans-retinoic acid.

Brefily introduce a protein function of the ABHD10?

It regulates the mitochondrial S-depalmitoylation of the nucleophilic active site residue of peroxiredoxin-5/PRDX5, a key antioxidant protein, therefore modulating mitochondrial antioxidant ability

How is the function of the ABDH10 when it act as a prognostic marker?

Prognostic marker in renal cancer (favorable) and cervical cancer (favorable).

Does the ABHD 10 protein can be detect in blood by proximity extension assay?

No

Please list at least three cellular compnents of the ABHD10?

Cell projection, cytoskeleton, and non-membrane-bounded organelle.

What is the name of ABHD10 inhibitor, which have function of increase the catalytic effciency of AcMPAG (mycophenolic acid acyl glucuronide) formation in human liver homogenate?

Phenylmethyl-sulfonyl fluride (PMSF).

What is the representative subtrate drug of ABHD10?

Mycophenolic acid acyl-glucurnide.

What is the proportion of amino acid homology does ABHD10 have between humans and rodents (mice and rats)?

Approximately 75%.

What biological process does ABHD10 regulate in human liver?

ABHD10 modulates Probenecid Acyl Glucuronidation in human liver.

ABHD10 plays a key role in the metabolism of the immunosuppressant mycophenolate mofetil (MMF), because it led to the deglucuronidation in human liver of acyl glucuronide metabolite (AcMPAG). What might this information suggest about the ABHD10 protein?

ABHD10 exerted a detoxifying effect.