Recombinant Human ABHD10 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ABHD10-5463H
• Product Overview: ABHD10 MS Standard C13 and N15-labeled recombinant protein (NP_060864) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a mitochondrially-localized enzyme that acts in liver cells as a hydrolase. The encoded protein removes glucuronide from mycophenolic acid acyl-glucuronide. There is a pseudogene for this gene on chromosome 6. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 33.9 kDa
• AA Sequence: MAVARLAAVAAWVPCRSWGWAAVPF GPHRGLSVLLARIPQRAPRWLPACR QKTSLSFLNRPDLPNLAYKKLKGKS PGIIFIPGYLSYMNGTKALAIEEFC KSLGHACIRFDYSGVGSSDGNSEES TLGKWRKDVLSIIDDLADGPQILVG SSLGGWLMLHAAIARPEKVVALIGV ATAADTLVTKFNQLPVELKKEVEMK GVWSMPSKYSEEGVYNVQYSFIKEA EHHCLLHSPIPVNCPIRLLHGMKDD IVPWHTSMQVADRVLSTDVDVILRK HSDHRMREKADIQLLVYTIDDLIDK LSTIVNTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ABHD10 abhydrolase domain containing 10 [ Homo sapiens (human) ]
• Official Symbol: ABHD10
• Synonyms: ABHD10; abhydrolase domain containing 10; abhydrolase domain-containing protein 10, mitochondrial; FLJ11342
• Gene ID: 55347
• mRNA Refseq: NM_018394
• Protein Refseq: NP_060864
• MIM: 618756
• UniProt ID: Q9NUJ1

Reviews

08/28/2019

Suitable price, great!

03/27/2018

Fast shipping and good product.

12/10/2017

Nice performance in electrophoresis.

07/12/2017

Good work in purification process!

Q&As

What kind of chemical compounds can interact with the ABHD10 protein?

 1,3-dinitrobenzene; 2,3,7,8-tetrachlorodibenzodioxine; all-trans-retinoic acid.

Brefily introduce a protein function of the ABHD10?

It regulates the mitochondrial S-depalmitoylation of the nucleophilic active site residue of peroxiredoxin-5/PRDX5, a key antioxidant protein, therefore modulating mitochondrial antioxidant ability

How is the function of the ABDH10 when it act as a prognostic marker?

Prognostic marker in renal cancer (favorable) and cervical cancer (favorable).

Does the ABHD 10 protein can be detect in blood by proximity extension assay?

No

Please list at least three cellular compnents of the ABHD10?

Cell projection, cytoskeleton, and non-membrane-bounded organelle.

What is the name of ABHD10 inhibitor, which have function of increase the catalytic effciency of AcMPAG (mycophenolic acid acyl glucuronide) formation in human liver homogenate?

Phenylmethyl-sulfonyl fluride (PMSF).

What is the representative subtrate drug of ABHD10?

Mycophenolic acid acyl-glucurnide.

What is the proportion of amino acid homology does ABHD10 have between humans and rodents (mice and rats)?

Approximately 75%.

What biological process does ABHD10 regulate in human liver?

ABHD10 modulates Probenecid Acyl Glucuronidation in human liver.

ABHD10 plays a key role in the metabolism of the immunosuppressant mycophenolate mofetil (MMF), because it led to the deglucuronidation in human liver of acyl glucuronide metabolite (AcMPAG). What might this information suggest about the ABHD10 protein?

ABHD10 exerted a detoxifying effect.