Recombinant Human ABHD13 293 Cell Lysate

• Cat.No.: ABHD13-9138HCL

Specification

• Description: Antigen standard for abhydrolase domain containing 13 (ABHD13) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ABHD13 abhydrolase domain containing 13 [ Homo sapiens ]
• Official Symbol: ABHD13
• Synonyms: BEM46L1; C13orf6; bA153I24.2; RP11-153I24.2
• Gene ID: 84945
• mRNA Refseq: NM_032859.2
• Protein Refseq: NP_116248.2
• UniProt ID: Q7L211
• Chromosome Location: 13q33.3
• Function: hydrolase activity

Reviews

01/10/2022

The product helped me get the experimental results that I was looking for, prefect!

10/13/2021

Good quality products.

09/11/2021

Nice performance in WB analysis!

Q&As

List at least three biological process of the ABHD13?

1. RNA metabolic process. 2. immune system process. 3. cell differentiation

How does the ABHD13 influence the mice?

It has also been reported that ABHD13 is regulated by circadian rhythm in rat liver 

What is the proper buffer solution for the ABHD13 protein?

50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

What disease occurrence related to ABHD13?

Diseases associated with ABHD13 include Ascaris Lumbricoides Infection and Williams-Beuren Syndrome

Where does the antigen of ABHD13 derived from?

The antigent from E. coli in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, etc..

Which organs in mice have higher expression of the ABHD13?

In mice, ABHD13 mRNA is very abundantly and ubiquitously expressed, with highest levels found in testes and the distal small intestine.

What size of the ABHD13 antibody when apply the western blot analysis using NIH-3T3 whole cell lysates?

The size is around 40kDa.

How does the ABHD13 perform when cancer cells were found in human body?

ABHD13 is upregulated in the early response to chemotherapy treatment in human ovarian cancer cell lines

Why the ABHD13 is not expected to possess acyltransferase activity?

It is bececause ABHD13 lacks a HX4D motif.

What is the storage condition of the ABHD13?

Store at -20 degree C. Stable for 12 months from date of receipt.