Recombinant Human ABHD14A 293 Cell Lysate

• Cat.No.: ABHD14A-9137HCL

Specification

• Description: Antigen standard for abhydrolase domain containing 14A (ABHD14A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ABHD14A?abhydrolase domain containing 14A [?Homo sapiens?(human) ]
• Official Symbol: ABHD14A
• Synonyms: ABHD14A; abhydrolase domain containing 14A; DORZ1; alpha/beta hydrolase domain-containing protein 14A; abhydrolase domain-containing protein 14A
• Gene ID: 25864
• mRNA Refseq: NM_015407
• Protein Refseq: NP_056222
• UniProt ID: Q9BUJ0
• Chromosome Location: 3p21.1
• Function: hydrolase activity

Reviews

12/01/2020

I will repurchase it for next lab seesion!

01/16/2019

Customer service patiently answers after-sales questions.

09/29/2018

The WB image of ABHD14A antibody is quite similar as the instruction displayed.

07/07/2018

Delivery was fast!

Q&As

Does the ABHD14A gene can be detected in blood by mass spectrometry?

Yes

List some other proteins which interacte with the ABHD14A in a hydrolase activity?

The related protein included: DPEP3, CTDSPL2A, PXYLP1, and MCM6, etc.

What disease would be influenced by ABHD14A?

Diseases associated with ABHD14A include Chanarin-Dorfman Syndrome and Autosomal Recessive Intellectual Developmental Disorder

Describe te stability of the ABHD14A ?

Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

What is the function of ABHD14A when it act as protein?

Possible role in granule neuron development.

What is the molecular function of the ABHD14A gene?

It is hydrolase activity

What is the suggested antigen peptide sequences for ABHD14A gene?

Design optimal peptide antigens: Abhydrolase domain-containing protein 14A (ABHEA_HUMAN)

What is the purity of the ABHD14A protein?

>80% by SDS-PAGE and Coomassie blue staining

How to prepare the ABHD14A?

Recombinat protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

What is the potenital source of the ABHD14A?

ABHD14A (T-14) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an internal region of ABHD14A of mouse origin.

What is the expected buffer solution for the ABHD14A?

Phosphate-buffered saline (PBS) and 1M Urea, pH 7.4.