Recombinant Human ABHD15, His-tagged

• Cat.No.: ABHD15-9239H
• Product Overview: Recombinant human ABHD15 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Protein length: 125-468a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: ABHD15 abhydrolase domain containing 15 [ Homo sapiens ]
• Official Symbol: ABHD15
• Synonyms: ABHD15; abhydrolase domain containing 15; abhydrolase domain-containing protein 15
• Gene ID: 116236
• mRNA Refseq: NM_198147
• Protein Refseq: NP_937790
• UniProt ID: Q6UXT9
• Chromosome Location: 17q11.2
• Function: carboxylesterase activity; hydrolase activity

Reviews

10/09/2021

I have bought it for twice, nice!

05/01/2021

The in-person customer service provide clear expression for both Pre-sale and after-sale!

11/16/2020

The purification rsults demonstrtaed clearly! Great.

11/15/2020

Prefect product and lovely serivces.

Q&As

What is the interaction between ABHD15 and PDE3B?

The expression of ABHD15 is induced during 3T3-L1 adipocyte differentiation and ABHD15 knock down in 3T3-L1 adipocytes resulted in loss of the PDE3B protein.

What biological technique can be used to detect the interaction between ABHD15 and PDE3B?

Using an antibody directed against the whole protein sequence, and the results confirmed in various cell lines that ABHD15 co-immunoprecipitates with PDE3B. Within this complex, ABHD15 likely regulates PDE3B expression and/or protein stability, as its expression is also impaired in the WAT of ABHD15-Knockout mice.

How does the ABHD15 expression?

ABHD15 protein was mainly expressed in 3T3-L1 adipocytes and adipose depots, followed by a weak expression in liver and pancreas.ABHD15 was not detected in skeletal muscle (SM) and cardiac muscle (CM)

Why does ABHD15 play a role in adipocyte development?

Because the ABHD15 is mainly expressed in murine adipose tissues and upregulated during in vitro adipogenesi; Additionally, ABHD15 expression is required for adipogenesis since Abhd15-silenced 3T3-L1 cells were unable to increase the expression levels of adipogenic marker genes, leading to reduced lipid accumulation.

What laboratory technology used for purification of ABHD15 protein?

Affinity chromatography.

List at least three biological process of the ABHD15?

1. Homeostatic process 2. Programmed cell death 3. Lipid metabolic process

What is the difference between ABHD15 and other proteins belong to ABHD family?

Typically, ABHD proteins possess a catalytic triad consisting of a nucleophile (Ser, Cys, or Asp), an acid (aspartate or glutamate), and a conserved histidine residue enabling hydrolase activity, whereas the ABHD15 does not contain a nucleophile.

What is the buffer solution of the ABHD15 protein?

Prior to lyophilization: 1% BSA and 0.02% NaN3.

What kind of evidence suggested that the ABHD15 protein play a critical role in insulin-regulated lipolysis?

Abhd15 is highly expressed in ATs. Further, Abhd15 expression was decreased in white adipose tissue (WAT) of aged and obese mice and in differentiated 3T3-L1 cells upon lipolytic stimulation

What are the recommended dilutions of the ABHD15 for diferent tested applications?

ELISA: 1/20000 - 1/80000, WB: 1/200 - 1/1000.