Recombinant Human ABHD3 293 Cell Lysate

• Cat.No.: ABHD3-9133HCL

Specification

• Description: Antigen standard for abhydrolase domain containing 3 (ABHD3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ABHD3 abhydrolase domain containing 3 [ Homo sapiens ]
• Official Symbol: ABHD3
• Synonyms: ABHD3; abhydrolase domain containing 3; abhydrolase domain-containing protein 3; LABH3; lung alpha/beta hydrolase 3; alpha/beta hydrolase domain containing protein 3; MGC11259
• Gene ID: 171586
• mRNA Refseq: NM_138340
• Protein Refseq: NP_612213
• MIM: 612197
• UniProt ID: Q8WU67
• Chromosome Location: 18q11.1
• Function: carboxylesterase activity; hydrolase activity; molecular_function

Reviews

06/06/2017

I have been using your company's protein products and hope to maintain the high quality in the future.

04/05/2016

The recombinant protein has excellent quality and reliability, saving me a lot of time and hassle.

Q&As

Can you provide antibodies for Abhd3 protein?

You can describe your experimental needs in detail to our scientists (WB, IF, IP, IHC, etc.) and they will provide professional solutions.

What is the purity of Recombinant Human ABHD3, His-tagged (ABHD3-9241H)?

Greater or equal to 85% purity as determined by SDS-PAGE. And you can also choose the required purity according to the application.

I have purchased your Abhd3 recombinant protein, how do I ensure long-term storage?

Please store the protein solution under the conditions recommended in the product manual.

Can you provide human ABHD3 recombinant protein with different truncations?

Of course, we offer custom protein production services, and you can describe in detail the protein length that meets your requirements when submitting your RFQ.

I browsed your ABHD3 product list, but I didn't find the fusion protein with the desired tag?

We support custom production of recombinant proteins, and you can choose the type and position of the tag you need when submitting the online inquiry form.

What biological processes is Abhd3 involved in?

It may be involved in lipid metabolism and phospholipid metabolism.

Is there any discount for ABHD3 recombinant protein?

We are offering a 10% discount on all products for a limited time. The clock is ticking down. Be sure to take advantage of our holiday promotions!