Recombinant Human ABI1 protein, His & T7-tagged

• Cat.No.: ABI1-1704H
• Product Overview: Recombinant Human ABI1 aa. (Met1~Pro182 (Accession # Q8IZP0)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interactions with Abelson tyrosine kinases. The encoded protein plays a role in macropinocytosis as a component of the WAVE2 complex, and also forms a complex with EPS8 and SOS1 that mediates signal transduction from Ras to Rac. This gene may play a role in the progression of several malignancies including melanoma, colon cancer and breast cancer, and a t(10;11) chromosomal translocation involving this gene and the MLL gene has been associated with acute myeloid leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 14.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 24.3kDa
• Protein length: Met1~Pro182 (Accession # Q8IZP0)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 9.5

Gene Information

• Gene Name: ABI1 abl interactor 1 [ Homo sapiens (human) ]
• Official Symbol: ABI1
• Synonyms: ABI1; abl interactor 1; E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1; Abelson interactor 1; Abl-interactor protein 1 long; abl-binding protein 4; eps8 SH3 domain-binding protein; interactor protein AblBP4; nap1 binding protein; spectrin SH3 domain-binding protein 1
• Gene ID: 10006
• mRNA Refseq: NM_001012750.2
• Protein Refseq: NP_001012768.1
• UniProt ID: Q8IZP0

Reviews

06/14/2020

Recombinant chicken ABI1 has high purity and good immunogenicity, and the experimental results are very satisfactory.

05/13/2018

The technical support experts at Creative BioMart customized the ABI1 protein with a specific expression system and fusion tag for me.

Q&As

Is there a TEV cleavage site between the GST tag and the ABI1 protein sequence?

Some of our products contain cleavage sites between the tag and the target protein. Please consult our experts for specific product information.

Can you express the ABI1 fusion protein with MBP tag?

Sure, Creative BioMart supports custom protein production services.

Which expression systems does recombinant ABI1 protein use?

We provide various expression systems, including E. coli, wheat germ, in vitro cell free system, and mammalian cell expression systems.

What signaling pathway is ABI1 involved in?

ABI1 may play a role in regulating EGF-induced activation of the Erk pathway and, together with EPS8, is involved in signal transduction from Ras to Rac.

Can your ABI1 proteins be used for WB?

Yes, our recombinant ABI1 protein can be used as a variety of applications based on the antigen-antibody reaction.