Recombinant Human ABI1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ABI1-4188H
• Product Overview: ABI1 MS Standard C13 and N15-labeled recombinant protein (NP_005461) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interactions with Abelson tyrosine kinases. The encoded protein plays a role in macropinocytosis as a component of the WAVE2 complex, and also forms a complex with EPS8 and SOS1 that mediates signal transduction from Ras to Rac. This gene may play a role in the progression of several malignancies including melanoma, colon cancer and breast cancer, and a t(10;11) chromosomal translocation involving this gene and the MLL gene has been associated with acute myeloid leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 14.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 55.1 kDa
• AA Sequence: MAELQMLLEEEIPSGKRALIESYQN LTRVADYCENNYIQATDKRKALEET KAYTTQSLASVAYQINALANNVLQL LDIQASQLRRMESSINHISQTVDIH KEKVARREIGILTTNKNTSRTHKII APANMERPVRYIRKPIDYTVLDDVG HGVKWLKAKHGNNQPARTGTLSRTN PPTQKPPSPPMSGRGTLGRNTPYKT LEPVKPPTVPNDYMTSPARLGSQHS PGRTASLNQRPRTHSGSSGGSGSRE NSGSSSIGIPIAVPTPSPPTIGPAP GSAPGSQYGTMTRQISRHNSTTSST SSGGYRRTPSVTAQFSAQPHVNGGP LYSQNSIADSPTPPPPPPPDDIPMF DDSPPPPPPPPVDYEDEEAAVVQYN DPYADGDPAWAPKNYIEKVVAIYDY TKDKDDELSFMEGAIIYVIKKNDDG WYEGVCNRVTGLFPGNYVESIMHYT DTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ABI1 abl-interactor 1 [ Homo sapiens (human) ]
• Official Symbol: ABI1
• Synonyms: ABI1; abl-interactor 1; spectrin SH3 domain binding protein 1, SSH3BP1; abl interactor 1; ABI 1; E3B1; Abelson interactor 1; nap1 binding protein; abl-binding protein 4; interactor protein AblBP4; Abl-interactor protein 1 long; eps8 SH3 domain-binding protein; spectrin SH3 domain-binding protein 1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1
• Gene ID: 10006
• mRNA Refseq: NM_005470
• Protein Refseq: NP_005461
• MIM: 603050
• UniProt ID: Q8IZP0

Reviews

06/14/2020

Recombinant chicken ABI1 has high purity and good immunogenicity, and the experimental results are very satisfactory.

05/13/2018

The technical support experts at Creative BioMart customized the ABI1 protein with a specific expression system and fusion tag for me.

Q&As

Is there a TEV cleavage site between the GST tag and the ABI1 protein sequence?

Some of our products contain cleavage sites between the tag and the target protein. Please consult our experts for specific product information.

Can you express the ABI1 fusion protein with MBP tag?

Sure, Creative BioMart supports custom protein production services.

Which expression systems does recombinant ABI1 protein use?

We provide various expression systems, including E. coli, wheat germ, in vitro cell free system, and mammalian cell expression systems.

What signaling pathway is ABI1 involved in?

ABI1 may play a role in regulating EGF-induced activation of the Erk pathway and, together with EPS8, is involved in signal transduction from Ras to Rac.

Can your ABI1 proteins be used for WB?

Yes, our recombinant ABI1 protein can be used as a variety of applications based on the antigen-antibody reaction.